抑制黄曲霉毒素合成的深海环状芽孢杆菌活性物质发酵条件优化  

Fermentation Optimization of Anti-aflatoxigenic Bioactive Metabolites Produced by a Deep-sea Bacillus circulus Strain

作　　者：王琪 闫培生 周莹 高秀君 王凯 贾文文 WANG Qi;YAN Peisheng;ZHOU Ying;GAO Xiujun;WANG Kai;JIA Wenwen(School of Marine Science and Technology,Harbin Institute of Technology（Weihai）,Weihai Shandong 264209,China)

机构地区：[1]哈尔滨工业大学(威海)海洋科学与技术学院,山东威海264209

出　　处：《中国农业科技导报》2018年第9期65-71,共7页Journal of Agricultural Science and Technology

基　　金：国际海域资源调查与开发"十二五"重大项目(DY125-15-R-01)资助

摘　　要：为了开发有效防控黄曲霉毒素污染的深海微生物农药,以淀粉酪蛋白培养基为基础,利用均匀设计,对一株能显著抑制黄曲霉毒素合成的深海环状芽孢杆菌活性物质的发酵培养基成分和发酵条件进行了优化。获得的优化方案为7.675 g/L淀粉、0.787 g/L酪蛋白、2.257 g/L酵母膏、57.91%海水用量,pH 4.15,1.02%的菌种接种量,培养温度20.51℃、培养时间3.28 d。与原始方案相比,优化方案的发酵培养基成本降低了42.76%,发酵温度由原来的28℃降低为20.51℃,培养时间由6 d减少到3.28 d。该优化方案具有明显的实用性和可操作性。In order to develop effective deep-sea microbial pesticide against aflatoxin contamination,this study optimized the fermentation medium composition and fermentation conditions of a deep-sea Bacillus circulus,showing significant inhibition activity against aflatoxin synthesis by uniform design based on starch-casein medium. The optimum scheme was consisted of 7.675 g/L starch,0.787 g/L casein,2.257 g/L yeast extract,57.91% seawater,4.15 pH,1.02% strain inoculation amount,20. 51℃ culture temperature,and 3. 28 d culture time. Compared with the original scheme,the cost of fermentation medium was reduced by 42.76%,fermentation temperature was reduced from 28℃ to 20.51℃,and culturing time was reduced from 6 d to 3.28 d. The optimized scheme established in this study had obvious practicability and maneuverability.

关 键 词：环状芽孢杆菌 发酵优化 黄曲霉毒素 

分 类 号：Q939.97[生物学—微生物学]