超声辅助乙醇提取铜藻中岩藻黄素的工艺研究  

Optimization of Ultrasonic Enhanced Extraction of Fucoxanthin from Sargassum horneri

作　　者：李红艳 刘天红 姜晓东 王颖 李大鹏 李晓 孙元芹 纪蕾 LI Hongyan;LIU Tianhong;JIANG Xiaodong;WANG Ying;LI Dapeng;LI Xiao;SUN Yuanqin;JI Lei(Marine Biology Institute of Shandong Province,Shandong Qingdao 266002,China)

机构地区：[1]山东省海洋生物研究院,山东青岛266002

出　　处：《中国农业科技导报》2018年第9期146-153,共8页Journal of Agricultural Science and Technology

基　　金：海洋公益性行业科研专项(201505022-4)资助

摘　　要：对超声辅助有机溶剂法提取鲜铜藻Sargassum horneri中岩藻黄素的提取工艺进行研究。采用高效液相色谱法(high performance liquid chromatography,HPLC)检测岩藻黄素含量,以单因素实验考察了提取溶剂、乙醇浓度、超声时间、料液比、提取温度和提取次数对岩藻黄素提取率的影响,并通过正交试验优化得到最佳工艺。提取的最佳工艺为:乙醇浓度90%,超声时间18 min,料液比1 g∶5 mL,提取温度45℃,提取2次。此条件下岩藻黄素提取率达到0.292 5 mg/g鲜重(1.460 3 mg/g干重)。鲜铜藻中岩藻黄素的含量十分丰富,超声辅助乙醇提取岩藻黄素提取效率高、方便快捷。This paper studied the technology of fucoxanthin extraction from Sargassum horneri assisted by ultrasonic.The content of fucoxanthin was analyzed by high performance liquid chromatography（ HPLC）. Single factor experiments were conducted to investigate the factors affecting extraction rate of fucoxanthin,including solvent,ethyl alcohol concentration,ultrasound time,solid-liquid ratio,extraction temperature and extraction times. The optimized technology was obtained by orthogonal test. The optimum extraction technology included 90% ethanol concentration,18 min ultrasound time,solid-liquid ratio 1 g ∶5 mL,extraction temperature 45℃ and extract time twice. Under these conditions,the extraction rate of fucoxanthin was 0.292 5 mg/g FW（ 1.460 3 mg/g DW）. The fucoxanthin content in fresh Sargassum horneri was very rich. The extraction rate of fucoxanthin with ethyl alcohol assisted by ultrasonic was high,and the method was easy and simple to handle.

关 键 词：铜藻 岩藻黄素 超声辅助 工艺优化 

分 类 号：S985.4[农业科学—捕捞与储运]