microRNA-196b、microRNA-217与TGFβR1蛋白在胰腺导管腺癌组织中的表达及其意义  

The expressions of microRNA-196b, microRNA-217, and TGFβR1 protein in the pancreatic ductal adenocarcinoma tissues

作　　者：苗春木[1] 龚建平[1] 熊彬[1] 游科[1] 邓开 MIAO Chunmu;GONG Jianping;XIONG Bin;YOU Ke;DENG Kai(Department of Hepatobiliary Surgery,The Second Affiliated Hospital of Chongqing Medical University,Chongqing 400010,P.R.China)

机构地区：[1]重庆医科大学附属第二医院肝胆外科,重庆400010

出　　处：《中国普外基础与临床杂志》2018年第9期1077-1082,共6页Chinese Journal of Bases and Clinics In General Surgery

摘　　要：目的检测胰腺导管腺癌组织中微小RNA(microRNA,miR)-196b、miR-217与转化生长因子β受体1(transforming growth factorβreceptor 1,TGFβR1)蛋白的表达水平,并探讨其临床意义。方法前瞻性收集2014年3月到2017年3月期间在重庆医科大学附属第二医院行胰腺癌根治术的30例胰腺导管腺癌患者的组织标本(包括胰腺导管腺癌组织及其癌旁组织),利用实时荧光定量聚合酶链反应法检测miR-196b和miR-217的表达水平,采用Western blotting法检测TGFβR1蛋白的表达水平。结果胰腺导管腺癌组织中miR-196b和TGFβR1蛋白的表达水平均高于癌旁组织(P<0.001),而miR-217的表达水平低于癌旁组织(P=0.001)。胰腺导管腺癌组织中miR-196b的表达水平与TGFβR1蛋白的表达水平呈正相关(r=0.803,P<0.001),而miR-217的表达水平与TGFβR1蛋白的表达水平呈负相关(r=–0.839,P<0.001)。结论胰腺导管腺癌组织中TGFβR1蛋白的表达可能同时受miR-196b和miR-217的双向调控,这种双向调控机制可能是制约胰腺导管腺癌发生和发展的重要机制之一,也可能是治疗胰腺导管腺癌的潜在靶点。Objective To determine the expression levels of micro RNA （miR）-196, miR-217, and transforming growth factor β receptor 1 （TGFβR1） protein in the pancreatic ductal adenocarcinoma tissues and its adjacent tissues, to reveal the relationship among them in the pathological process of pancreatic ductal adenocarcinoma. Methods A total of 30 cases’ pancreatic ductal adenocarcinoma tissues and its adjacent tissues were collected. The expression levels of miR-196b and miR-217 in the pancreatic ductal adenocarcinoma and adjacent tissues were detected by real-time fluorescence quantitative polymerase chain reaction method, the level of TGFβR1 protein was detected by Western blotting method. Results In the pancreatic ductal adenocarcinoma tissues, the expression levels of miR-196b and TGFβR1 protein were significantly higher than those of adjacent tissues （P〈0.001）, while the level of miR-217 was significantly lower than that of adjacent tissues （P=0.001）. For further detection, the level of miR-196b in pancreatic ductal adenocarcinoma tissues was significantly positively correlated with the expression level of TGFβR1 protein （r=0.803, P〈0.001）, while the expression level of miR-217 was negatively correlated with the expression level of TGFβR1 protein （r=–0.839, P〈0.001）. Conclusions Expression TGFβR1 protein in pancreatic ductal adenocarcinoma tissues may be bi-directionally regulated by miR-196b and miR-217. This two-way regulating mechanism may be one of the important mechanisms for restricting the development of pancreatic ductal adenocarcinoma, implying a potential target for treatment of pancreatic cancer.

关 键 词：胰腺导管腺癌 microRNA-196b、microRNA-217 转化生长因子β受体1 

分 类 号：R735.9[医药卫生—肿瘤]