Responsive graphene oxide hydrogel microcarriers for controllable cell capture and release  

智能响应性氧化石墨烯水凝胶微载体用于细胞的捕获与释放（英文）

作　　者：Jie Wang Guopu Chen Ze Zhao Lingyu Sun Minhan Zou Jian＇an Ren Yuanjin Zhao 王洁;陈国璞;赵泽;孙灵钰;邹旻含;任建安;赵远锦(State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University;Department of General Surgery, Jinling Hospital, Medical School of Nanjing University)

机构地区：[1]State Key Laboratory of Bioelectronics,School of Biological Science and Medical Engineering,Southeast University,Nanjing 210096,China [2]Department of General Surgery,jinling Hospital,Medical School of Nanjing University,Nanjing 210002,China

出　　处：《Science China Materials》2018年第10期1314-1324,共11页中国科学（材料科学（英文版）

基　　金：supported by the National Natural Science Foundation of China (21473029 and 51522302);the NSAF Foundation of China (U1530260);the Scientific Research Foundation of Southeast University;the Scientific Research Foundation of Graduate School of Southeast University;the Postgraduate Research & Practice Innovation Program of Jiangsu Province;the Fundamental Research Funds for the Central Universities

摘　　要：Cell microcarriers have emerged as a powerful cell culture platform in biomedical areas, but their functions are usually limited to simply capturing and proliferating cells,because of the simplicity of their components. Thus, in this study, we developed a new near-infrared（NIR） light-responsive graphene oxide（GO） hydrogel microcarrier system for controllable cell culture. The microcarriers were generated by using capillary microfluidics to emulsify the GO dispersed poly（N-isopropylacrylamide）（pNIPAM） and gelatin methacrylate（GelMA） pre-gel solution. The composite GO hydrogel microcarriers exhibited photothermally responsive cell capture, as well as the capacity for proliferation and release due to the NIR absorption of GO, the thermally responsive shape transition of pNIPAM, and the high biocompatibility of Gel MA. It was found that the NIR-responsive GO hydrogel microcarriers could prevent the cultured cells from being attacked by the immune system and promote the formation of tumor models in immunocompetent mice, which is desired for tumor and drug research. These features make the NIR-responsive GO hydrogel microcarriers excellent functional materials for different biomedical applications.细胞微载体作为生物医学领域中三维细胞培养的一个平台,通常会由于过于简单的材料组成成分,使得微载体的功能局限于细胞捕获与增殖.本研究开发了一种近红外光响应的氧化石墨烯水凝胶微载体用于细胞的可控培养.利用毛细管微流控技术,通过乳化氧化石墨烯—异丙基丙烯酰胺—甲基化明胶复合预聚溶液来制备智能响应性氧化石墨烯水凝胶微载体.氧化石墨烯对近红外光辐射的吸收能力,异丙基丙烯酰胺的热响应性相转换能力,以及甲基化明胶良好的生物相容性,使得该复合水凝胶微载体具有高效的光热响应的细胞捕获、增殖以及释放能力.此外,近红外光响应的氧化石墨烯水凝胶微载体还能够为其内部包裹的细胞提供保护,使其免受免疫系统的攻击,从而促进免疫活性小鼠体内肿瘤模型的形成与生长.

关 键 词：microfluidics graphene oxide MICROCARRIER HYDROGEL tumor 

分 类 号：R318.08[医药卫生—生物医学工程] TQ427.26[医药卫生—基础医学]