当归挥发油对人肺腺癌GLC-82细胞增殖及细胞周期的影响  

Effects of Volatile Oil from DangGui on Cell Cycle and Proliferation of Human Lung Adenocarcinoma GLC-82 Cells

作　　者：张正顺[1] 张艳霞 陈绍仪 孔学礼 周佳玮 ZHANG Zhengshun;ZHANG Yanxia;CHEN Shaoyi;KONG Xueli;ZHOU Jiawei(Baiyin Branch,Gansu Provincial Hospital of Traditional Chinese Medicine,Baiyin 730900,China;Gansu University of Chinese Medicine/Key Laboratory of Pharmacology and Toxicology for Traditional ChineseMedicines of Gansu Province（nurturing station）/ Key Laboratory of Chemistry and Quality for Traditional ChineseMedicines of the College of Gansu Province,Gansu University of Traditional Chinese Medicine;The Second People忆s Hospital of Baiyin City)

机构地区：[1]甘肃省中医院白银分院,甘肃白银730900 [2]甘肃中医药大学/甘肃省中药药理与毒理学重点实验室/甘肃省高校中(藏)药化学与质量研究省级重点实验室 [3]白银市第二人民医院

出　　处：《西部中医药》2018年第9期8-12,共5页Western Journal of Traditional Chinese Medicine

基　　金：白银市科技计划项目(编号2013-2-76Y);甘肃中医学院中青年科研基金项目(编号2305016601)

摘　　要：目的:探讨当归挥发油对人肺腺癌GLC-82细胞增殖及细胞周期的作用。方法:采用噻唑蓝(MTT)比色法检测当归挥发油对GLC-82细胞存活率的影响;倒置显微镜下观察细胞生长形态;流式细胞仪检测当归挥发油对GLC-82细胞周期的影响。结果:在6. 25～200μg/m L浓度范围内当归挥发油对GLC-82细胞增殖具有浓度依赖性抑制作用,当归挥发油对GLC-82细胞的I C50为113. 03μg/mL;倒置显微镜下观察发现阴性组细胞具有GLC-82细胞典型的形态特征,多边形,成片生长,随着当归挥发油浓度的升高,细胞数量减少,散在生长,形态发生变化,逐渐皱缩;经当归挥发油处理后GLC-82细胞表现出明显的G2期周期阻滞。结论:当归挥发油可抑制GLC-82细胞生长,引起细胞周期G2期的阻滞。Objective： To discuss the effects of volatile oil from DangGui （Angelica sinensis） on cell cycle andthe proliferation of human lung adenocarcinoma GLC-82 cells. Methods： The effects of volatile oil from DangGui onsurvival rate of GLC-82 cells were detected by using MTT chromometry; cell growth morphology was observedunder the inverted microscope; the influence of GLC-82 cell cycle was measures by flow cytometry. Results： Volatileoil from DangGui inhibited the proliferation of GLC-82 cells in concentration-dependent manner rangedbetween 6.25~200 μg/mL, IC50 of volatile oil from DangGui to GLC-82 cells was 113.03 μg/mL; the results underthe inverted microscope showed that the cells in negative group presented typical morphological features of GLC-82cells, polygon, flaky growth, as the concentrations of volatile oil from DangGui raised, cellular numbers reduced,spread in growth, the shape has changed and shrunk gradually; the results of flow cytometry indicated that GLC-82cells performed the obvious cell cycle arrest at phase G2 after dealing with volatile oil from DangGui. Conclusion：Volatile oil from DangGui could inhibit the growth of GLC-82 cells and cause cell cycle arrest at phase G2.

关 键 词：人肺腺癌GLC-82细胞 细胞周期 当归挥发油 

分 类 号：R734[医药卫生—肿瘤]