2014年～2016年山东省猪嵴病毒流行病学调查  

Epidemiological investigation of porcine Kobuvirus in Shandong province from 2014 to 2016

作　　者：王玮 刘海源 李鹏飞 宋莎莎 张瑞华[1] 陈君豪[1] 蓝晶晶 谢之景[1] 姜世金[1] WANG Wei;LIU Hai-yuan;LI Peng-fei;SONG Sha-sha;ZHANG Rui-hua;CHEN Jun-hao;LAN Jing-jing;XIE Zhi-jing;JIANG Shi-jin(College of Veterinary Medicine of Shandong Agricultural University,Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention,Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention Tai＇an 271018,China)

机构地区：[1]山东农业大学动物医学院/山东省动物生物工程与疾病防治重点实验室/山东省畜禽疫病防控工程技术研究中心,山东泰安271018

出　　处：《中国预防兽医学报》2018年第8期670-674,共5页Chinese Journal of Preventive Veterinary Medicine

基　　金：山东省双一流建设项目(SYL2017YSTD11)

摘　　要：为了解猪嵴病毒(PKV)在山东省内的流行情况,本研究根据PKV的3D基因保守区域设计一对引物,对2014年冬~2016年冬来自山东省350份病料样品进行RT-PCR检测和统计分析。结果显示:在350份病料样品中,PKV阳性率为56%(196/350),阳性猪场占80%(24/30);非腹泻猪个体阳性率(65.38%,153/234)明显高于腹泻猪个体阳性率(37.07%,43/116);育肥猪的PKV阳性率(97.50%)远高于哺乳期仔猪(45.40%)、断奶期仔猪(52.75%)以及成年猪(66.67%);夏秋季节采集的样品PKV检出率(71.94%)明显高于冬春季节(45.50%)。统计PKV与PEDV、TGEV混合感染结果显示,虽然PKV在非腹泻猪群中单独感染率(61.11%,143/234)远高于腹泻猪群(18.97%,22/116),但非腹泻猪群PKV与PEDV和TGEV的混合感染率却明显低于腹泻猪群。对5份PKV阳性样品的3D基因测序分析显示,5份阳性样品该基因核苷酸同源性为90.8%~99.7%,与GenBank中的参考株之间的核苷酸同源性为88.8%~94.1%。上述研究结果表明在山东省范围内PKV感染与猪腹泻之间无必然关系,PKV的3D基因进化与病毒株的分离年代和地域无明显相关性。To investigate the prevalence of porcine Kobuvirus（PKV） in Shandong province, a total of 350 clinical samples,collected from Shandong area between the winter of 2014 to the winter of 2016, were detected by RT-PCR with a pair of primers based on the conserved region of PKV 3 D gene. The results showed that the PKV positive rates in the clinical samples and pig farms were 56%（196/350） and 80%（24/30）, respectively, and the PKV positive rate in diarrhea pigs（65.38% 153/234） was significantly higher than that in non-diarrhea pigs（37.07%, 43/116）. The detection rate of PKV in fattening pigs（97.50%） was much higher than these in sucking piglets（45.40%）, weaning piglets（52.75%）, and adulthood pigs（66.67%）, while the detection rate of PKV in the samples taken in summer and autumn（71.94%） was significantly higher than that in spring and winter（45.5%）.Sequencing analysis of PKV 3 D genes showed that the nucleotide homology between the five PKVs in this study was 90.8%-99.7%,while the nucleotide homology between the five PKVs and the reference strains in GenBank was 88.8%-94.1%. The results suggested there was no inevitable relationship between PKV infection and swine diarrhea in Shandong area, and there was no significant correlation between the 3 D gene evolution and the isolated time or region of PKVs.

关 键 词：猪嵴病毒 3D基因 RT-PCR 流行病学调查 

分 类 号：S852.65[农业科学—基础兽医学]