非小细胞肺癌和肺炎性病变摄取18F－FDG与Glut－1、Glut－3、HK－Ⅱ表达的相关性  

Correlation of Glut-1, Glut-3 and HK-Ⅱ expression with 18F-FDG uptake in non-small cell lung cancer and pulmonary inflammatory lesions

作　　者：王振光[1] 于明明[1] 杨光杰[1] 韩瑜 王洋洋 赵鹏[2] Wang Zhenguang;Yu Mingming;Yang Guangjie;Han Yu;Wang Yangyang;Zhao Peng(PET/CT Center,the Affiliated Hospital of Qingdao University,Qingdao 266100,China;Department of Nuclear Medicine,Cangzhou Central Hospital,Cangzhou 061000,China;Department of Nuclear Medicine,the Second Hospital of Shandong University,Jinan 250033,China;Department of Pathology,the Affiliated Hospital of Qingdao University,Qingdao 266100,China)

机构地区：[1]青岛大学附属医院PET/CT中心,266100 [2]青岛大学附属医院病理科,266100 [3]河北省沧州市中心医院核医学科,061000 [4]山东大学第二医院核医学科,济南250033

出　　处：《中华核医学与分子影像杂志》2018年第9期605-608,共4页Chinese Journal of Nuclear Medicine and Molecular Imaging

摘　　要：目的分析非小细胞肺癌（NSCLC）和肺炎性组织中葡萄糖转运蛋白（Glut）－1、Glut－3、己糖激酶（HK）－Ⅱ的表达，探讨其与病变组织摄取18F－脱氧葡萄糖（FDG）的相关性。方法回顾性分析2012年11月至2016年5月间于青岛大学附属医院行18F－FDG PET/CT显像并经手术病理证实的22例肺炎性病变患者和24例NSCLC患者（男25例、女21例，年龄37～81岁），测定其病灶最大标准摄取值（SUVmax），采用免疫组织化学方法测定Glut－1、Glut－3、HK－Ⅱ表达，计算其染色总分并行半定量分析。采用单因素方差分析、最小显著差异t检验、两样本t检验和Spearman相关分析处理数据。结果24例NSCLC患者病灶SUVmax为8.71±7.62；Glut－1、Glut－3、HK－Ⅱ染色总分平均值分别为（3.75±0.99）、（4.04±1.00）和（4.00±0.78）分。22例肺炎性病变患者病变病灶SUVmax为3.29±2.16；Glut－1、Glut－3、HK－Ⅱ染色总分平均值分别为（2.32±0.65）、（2.89±0.83）和（2.41±0.50）分。肿瘤组织SUVmax高于炎性组织SUVmax（t＝3.220, P〈0.05），肿瘤组织Glut－1、Glut－3、HK－Ⅱ表达亦高于炎性组织相应指标（t值：5.340、5.160和8.130,均P〈0.01）。肿瘤组织Glut－1、HK－Ⅱ表达与SUVmax呈正相关（rs值：0.414和0.457，均P〈0.05）；炎性组织Glut－1、Glut－3、HK－Ⅱ表达与SUVmax无相关性（rs值：0.392、0.070和－0.066，均P〉0.05），但炎性组织Glut－3表达高于Glut－1和HK－Ⅱ（F＝4.123，t值：0.970和0.150，均P〈0.05）。结论Glut－1、Glut－3、HK－Ⅱ在NSCLC和肺炎性组织中均有表达，在NSCLC表达强度更高；Glut－1、HK－Ⅱ表达是NSCLC摄取18F－FDG的重要因素；Glut－3表达在肺炎性组织摄取18F－FDG中可能发挥更重要的作用。ObjectiveTo analyze the expression of glucose transport protein （Glut）-1, Glut-3 and hexokinase （HK）-Ⅱ in non-small-cell lung cancer （NSCLC） lesions and pulmonary inflammatory lesions and discuss the correlation of them with 18F-fluorodeoxyglucose （FDG） uptake.MethodsTwenty-four patients with NSCLC and 22 patients with pulmonary inflammatory lesions （25 males, 21 females; age range： 37-81 years） who underwent PET/CT from November 2012 to May 2016 were retrospectively analyzed. All patients had surgery and were confirmed by pathology. The expression of Glut-1, Glut-3 and HK-Ⅱ in the lesions was detected by immunohistochemistry. Immunohistochemical staining scores and maximum standardized uptake value （SUVmax） were calculated. One-way analysis of variance, the least significant difference t test, two-sample t test and Spearman correlation analysis were used.ResultsThe SUVmax of NSCLC lesions was 8.71±7.62, higher than that of pulmonary inflammatory lesions （3.29±2.16; t=3.220, P〈0.05）. Immunohistochemical staining scores of Glut-1, Glut-3 and HK-Ⅱ were 3.75±0.99, 4.04±1.00 and 4.00±0.78 for NSCLC lesions respectively, and were all higher than those of pulmonary inflammatory lesions （2.32±0.65, 2.89±0.83, 2.41±0.50; t values： 5.340, 5.160, 8.130, all P〈0.01）. The expression of Glut-1 and HK-Ⅱ was positively correlated with SUVmax in NSCLC lesions （rs values： 0.414, 0.457, both P〈0.05）. The expression of Glut-1, Glut-3 and HK-Ⅱ was not correlated with SUVmax （rs values： 0.392, 0.070, -0.066, all P〉0.05）, but the expression of Glut-3 was higher than that of Glut-1 and HK-Ⅱ （F=4.123, t values： 0.970, 0.150, all P〈0.05） in pulmonary inflammatory lesions.ConclusionsThe expression of Glut-1, Glut-3 and HK-Ⅱ is higher in NSCLC lesions than that in pulmonary inflammatory lesions. Glut-1 and HK-Ⅱ are the important factors for 18F-FDG uptake in NSCLC. Glut-3 may play an important role in 18F-FDG uptake in pulmonary inflammatory lesions.

关 键 词：癌 非小细胞肺 葡萄糖转运蛋白质类 易化性 己糖激酶 正电子发射断层显像术 体层摄影术 X线计算机 脱氧葡萄糖 

分 类 号：R734.2[医药卫生—肿瘤]