TLR2促进非小细胞肺癌细胞增殖及其作用机制  

TLR2 promotes cell proliferation in non-small cell lung cancer and its mechanism

作　　者：刘妍妍[1] 崔心明[2] 刘晓华[2] 王然[1] 杨永滨[1] 崔丹[1] 刘晓霞[3] LIU Yan-yan;CUI Xin-ming;LIU Xiao-hua;WANG Ran;YANG Yong-bin;CUI Dan;LIU Xiao-xia(Department of Physiology,Harbin Medical University,Harbin 150081,China;Department of Otolaryngology,Heilongjiang Provincial Hospital;Department of Cardiology,The Fourth Affiliated Hospital of Harbin Medical University,Harbin 150001,China)

机构地区：[1]哈尔滨医科大学生理学教研室,黑龙江哈尔滨150081 [2]黑龙江省医院南岗院区耳鼻喉科 [3]哈尔滨医科大学附属第四医院心内科,黑龙江哈尔滨150001

出　　处：《哈尔滨医科大学学报》2018年第3期212-215,共4页Journal of Harbin Medical University

基　　金：黑龙江省卫生计生委科研课题(2014-413)

摘　　要：目的研究TLR2(Toll-like receptor 2,TLR2)基因对非小细胞肺癌(non-small cell lung cancer,NSCLC)细胞增殖能力的影响及其对ERK信号通路的调节作用。方法将人非小细胞肺癌细胞株A549细胞分为空白组、空载体组、TLR2基因过表达组,MTT法检测细胞增殖能力,Western blot法检测ERK1/2和p-ERK1/2蛋白表达水平。结果在A549细胞中,过表达TLR2基因可促进非小细胞肺癌细胞增殖并上调ERK1/2磷酸化水平; U0126能抑制过表达TLR2基因对非小细胞肺癌细胞增殖的促进作用。结论TLR2基因可通过调控ERK信号通路促进体外非小细胞肺癌细胞增殖。Objective To investigate the regulation of TLR2 on proliferation and ERK pathway in non-small cell lung cancer （NSCLC） cells. Methods A549 cells, the non-small cell lung cancer cell line, were treated with blank, PCMV-myc and PCMV-TLR2, respectively. Then, cell proliferation was test by MTT assay and ERK protein level was tested by Western blot. In addition, the two cell lines were treated with U0126, an ERK1/2 phosphorylation inhibitor. Results Cell proliferation was promoted and the p-ERK1/2 expression was up-regulated by overexpression of TLR2 in A549 cells. Meanwhile, U0126 treatment restrained the promotion of TLR2 on cell proliferation. Conclusion Overexpression of TLR2 promotes cell proliferation through ERK pathway in NSCLC.

关 键 词：非小细胞肺癌 TLR2 ERK 信号通路 细胞增殖 

分 类 号：R734.2[医药卫生—肿瘤]