层状纳米颗粒Laponite对成牙本质细胞矿化促进作用的研究  

Experimental study on the biomineralization of odontoblast-lineage cells promoted by the nanoplatelet laponite

作　　者：陈羽浓 苏俭生 CHEN Yu - nong;SU Jian - sheng(Department of Prosthodontics, School and Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai 200072, China)

机构地区：[1]同济大学口腔医学院.附属口腔医院口腔修复教研室上海牙组织修复与再生工程技术研究中心,上海200072

出　　处：《牙体牙髓牙周病学杂志》2018年第9期497-503,共7页Chinese Journal of Conservative Dentistry

基　　金：国家自然科学基金(81572114);上海市科学技术委员会项目(17140903600)

摘　　要：目的:研究层状纳米粒颗粒Laponite对成牙本质细胞(OLCs)的矿化促进作用。方法:采用透射电镜(TEM)和动态光散射法(DLS)对Laponite进行形态表征观察;通过CCK-8和活/死细胞染色法检测不同浓度的Laponite对OLCs增殖和活力的影响,并分别通过划痕实验比较各组细胞的迁移能力,q-PCR检测各组细胞成牙本质相关基因的表达水平,茜素红染色和半定量分析比较各组细胞的钙化结节形成情况。结果:Laponite在水溶液中可分散形成纳米圆盘状颗粒,其平均粒径为(30. 9±7. 4) nm;当Laponite浓度小于100μg/mL时,对OLCs的增殖和活力无显著影响(P> 0. 05);而10μg/mL和100μg/mL的Laponite均能明显增强OLCs的迁移能力,并显著提高其成牙本质相关基因的表达水平以及钙化结节的形成量(P <0. 05)。结论:10μg/mL和100μg/mL的Laponite能够促进OLCs的生物矿化。AIM： To investigate the biomineralization of the nanoplatelet Laponite on odontoblast-lineage cells in vitro. METHODS： The morphology of Laponite was characterized by TEM and DLS. The effects of Laponite on cell proliferation and viability were examined by CCK - 8 and LIVE/DEAD cell staining. The cell migration rate was observed by wound healing assay. q-PCR was used to analyze the expression of the dentinogenesis-related genes （COL - 1, DSPP, DMP1 and BMP2）. Calcium accumulation was detected by using alizarin red staining and cetylpyridinium chloride assay. RESULTS： Laponite dispersed in aqueous solution and formed nanoplatelets of which particle size was （30.9±7.4）nm. When the concentration of Laponite was less than 100 μg/mL, cell proliferation and viability were not affected（ P 〉0.05）.The cell migration, expression of the dentinogenesis-related genes and calcium accumulation were promoted by Laponite at 10 μg/mL and 100 μg/mL（ P 〈0.05）. CONCLUSION： Laponite promotes the biomineralization of the OLCs.

关 键 词：LAPONITE 成牙本质细胞 生物矿化 修复性牙本质 

分 类 号：R780.2[医药卫生—口腔医学]