高产二十碳五烯酸的微拟球藻藻株的诱变选育  

Improvement of the eicosapentaenoic acid yield in Nannochloropsis using a new breeding strategy

作　　者：李悦明 崔球[2] 夏修峦 宋晓金[2] 徐建春 李霞 范栋 李妍 LI Yueming;CUI Qiu;XIA Xiuluan;SONG Xiaojin;XU Jianchun;LI Xia;FAN Dong;LI Yan(Qingdao Langyatai Group Co.,Ltd.,Qingdao 2GG400,China;Qingdao Institute of Bioenergy and Bioprocess Technology Chinese Academy of Sciences,Qingdao 2GG101,China;Huangdao Entry Exit Inspection and Quarantine Bureau,Qingdao 2GG101,China;Huangdao Drug Supervision and Administration Bureau,Qingdao 2GG400,China)

机构地区：[1]青岛琅琊台集团股份有限公司,山东青岛266400 [2]中国科学院青岛生物能源与过程研究所,山东青岛266101 [3]黄岛出入境检验检验局,山东青岛266400 [4]青岛市黄岛区食品药品监督管理局,山东青岛266400

出　　处：《发酵科技通讯》2018年第3期189-192,共4页Bulletin of Fermentation Science and Technology

基　　金：工信部绿色制造系统集成项目(Z135060009002);青岛市自主创新重大专项(16-7-1-11-zdzx-nsh)

摘　　要：二十碳五烯酸(C20∶5,n-3,EPA),是一种ω-3多不饱和脂肪酸,具有广泛的生物活性.目前,微拟球藻(Nannochloropsis)被认为是最有潜力的EPA的生产藻株而被广泛关注.针对微拟球藻野生藻株进行了重离子束辐照联合三氯生诱变选育,以期获得高产EPA的微拟球藻藻株.最终获得的突变株M44,其EPA质量分数为43.3%,EPA质量浓度达到2.5g/L,是出发藻株的2.38倍.本研究证实了一种可用于改良EPA生产的诱变育种策略,并提供了一种具有较高EPA产量的微拟球藻突变藻株.Eicosapentaenoic acid （C20： 5, n-3, EPA） is an omega 3 polyunsaturated fatty acid with wide biological activities. Currently, Nannochloropsis is considered to be the most potential EPA producer. In this study, we carried out a new selective breeding experiment of heavy ion beam irradiation combined with triclosan treatment, aiming to obtain the high EPA yield mutant of Nannochloropsis. Mutant strain M44 was obtained with EPA content of 43.3 %, and the EPA yield reached 2.5 g/L, which was 2.38 times higher than that of the original strain SD595. This study confirmed a feasible breeding strategy for improving EPA production, and provided a Nannochloropsis mutant with high EPA yield.

关 键 词：二十碳五烯酸 脂肪酸合酶 重离子诱变 微拟球藻 

分 类 号：Q815[生物学—生物工程]