辛芍组方中5个指标成分在大鼠体内的组织分布情况  

Tissue Distribution of Five Components from Xinshao Formula in Rats

作　　者：周孟[1] 胡贺佳 李梅[1] 李莹 薛寸 李月婷[1] 胡杰[1] 黄勇[1] 王广成 郑林[1] 李勇军[1] 王爱民[1] 席晓岚[1] 巩仔鹏[1] ZHOU Meng;HU He-jia;LI Mei;LI Ying;XUE Cun;LI Yue-ting;HU Jie;HUANG Yong;WANG Guang-cheng;ZHENG Lin;LI Yong-jun;WANG Ai-min;XI Xiao-lan;GONG Zi-peng(Provincial Key Laboratory of Pharmaceutics in Guizhou Province,State Key Laboratory of Functions and Applications of Medicinal Plants,Engineering Research Center for Development and Application of Ethnic Medicine and Traditional Chinese Medicine,Ministry of Education,School of Pharmaceutical Sciences,Guizhou Medical University,Guiyang 550004,China)

机构地区：[1]贵州医科大学贵州省药物制剂重点实验室药用植物功效与利用国家重点实验室民族药与中药开发应用教育部工程研究中心药学院

出　　处：《中国实验方剂学杂志》2018年第19期92-98,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81260636);贵州省优秀青年科技人才培养项目(黔科合平台人才[2017]5601号);贵阳市科研创新团队项目(筑科合同[2017]30-29号);贵州省科技厅科技创新人才团队建设项目(黔科合平台人才[2016]5613);贵州省高层次创新型人才培养计划项目(黔科合平台人才[2016]5677)

摘　　要：目的：研究大鼠灌胃辛芍组方后其5个指标成分在体内的组织分布情况。方法：建立同时测定大鼠组织中灯盏甲素、灯盏乙素、灯盏乙素苷元、芍药苷、芍药内酯苷的UPLC-MS/MS,将辛芍组方灌胃给予SD大鼠（剂量25 g·kg-1）,分别于给药后20,90 min和12,24 h取其主要脏器和组织,采用UPLC-MS/MS测定各个时间点下5个指标成分在脏器和组织中的分布情况。结果：大鼠灌胃辛芍组方后,5个指标成分可广泛分布于大鼠各组织器官中。对于灯盏甲素,其质量浓度20 min时在肺和心中达到峰值,分别为48.2,59.8μg·L-1;90 min时在脑和小肠中达到峰值,分别为138.7,292.7μg·L-1;12 h时在肾和肌肉中达到峰值,为517.3,56.5μg·L-1;24 h时在肝脏中达到峰值,为352.9μg·L-1。对于灯盏乙素,其质量浓度20 min时在心中达到峰值,为38.3μg·L-1;90 min时在肺、脑、小肠和肾中达到峰值,分别为30.6,220.0,1 644.2,859.9μg·L-1;12 h时在肌肉中达到峰值,为86.9μg·L-1;24 h时在肝脏中达到峰值,为344.8μg·L-1。对于灯盏乙素苷元,其质量浓度90 min时在脑、小肠和肌肉中达到峰值,分别为875.4,52 468.0,5 114.7μg·L-1;12 h时在肺、心和肾中达到峰值,分别为609.7,1 718.6,1 736.5μg·L-1;24 h时在肝脏中达到峰值,为1 366.5μg·L-1。对于芍药苷,其质量浓度20 min时在肺、肝、肾和肌肉中达到峰值,分别为10 634.1,13 889.0,16 672.0,7 750.4μg·L-1;在90 min时,在心、小肠和脑中达到峰值,分别为6 851.1,870 563.9,7 474.0μg·L-1。对于芍药内酯苷,其质量浓度20 min时在肝和肾中达到峰值,分别为15 249.7,56 817.0μg·L-1,在90 min时,在肺、心、脑、小肠和肌肉中达到峰值,分别为4 211.5,4 425.3,4 631.3,631 871.5,5 673.6μg·L-1。结论：大鼠灌胃辛芍组方后,5个指标成分可迅速、广泛地分布在各组织器官中,但5个指标成分在各组织脏器中的达峰时间和分布存在一定差异,在肾脏和小肠中的分布最高,其Objective： To study on the tissue distribution of five components from Xinshao formula in rats after administered intragastrically. Method： UPLC-MS/MS for the simultaneous determination of apigenin-7-Oglucronide,scutellarin,scutellarin aglycone,paeoniflorin and albiflorin in rat tissues was established. Rats were administered intragastrically Xinshao formula at a dose of 25 g·kg-1,and main tissues and organs were collected from rats at 20,90 min and 12,24 h after administration. After pretreatment,the concentrations of apigenin-7-Oglucronide,scutellarin,scutellarin aglycone,paeoniflorin and albiflorin in each organ of each time point were determined. Result： After being administered Xinshao formula,five components could be widely distributed in tissues and organs of rats. For apigenin-7-O-glucronide,its concentration peaked in the lung and heart at 20 min with 48. 2,59. 8 μg·L-1; peaked in the brain and small intestine at 90 min with 138. 7,292. 7 μg·L-1; peaked in kidney and muscle at 12 h with 517. 3,56. 5 μg·L-1; peaked in the liver at 24 h with 352. 9 μg·L-1. For scutellarin,its concentration peaked in the heart at 20 min with 38. 3 μg·L-1; peaked in the lung,brain,small intestine and kidney at 90 min with 30. 6,220. 0,1 644. 2,859. 5 μg·L-1; peaked in the muscle at 12 h with86. 9 μg·L-1 and in the liver at 24 h with 344. 8 μg·L-1. For scutellarin aglycone,its concentration peaked in the brain,small intestine and muscle at 90 min with 875. 4,52 468. 0,5 114. 7 μg·L-1; peaked in lung,heart and kidney at 12 h with 609. 7,1 718. 6,1 736. 5 μg·L-1 and peaked in the liver at 24 h with 1 366. 5 μg·L-1.For paeoniflorin,its concentration reached peaks in the lung,liver,kidney and muscle at 20 min with 10 634. 1,13 889. 0,16 672. 0,7 750. 4 μg·L-1; and reached in the heart,small intestine and brain at 90 min with6 851. 1,870 563. 9,7 474. 0 μg·L-1. For albiflorin,its concentration peaked in the liver and kidney at 20 min with 15 249. 7,56 817. 0 μg·L-1; peaked in the lung,heart,brain

关 键 词：辛芍组方 灯盏乙素 灯盏甲素 芍药苷 组织分布 

分 类 号：R22[医药卫生—中医基础理论] R969.1[医药卫生—中医学]