金龙胶囊对胃癌细胞MGC-803和BGC-823侵袭转移能力的影响  

Effect of Jinlong Capsule on Invasion and Migration of Human Gastric Carcinoma MGC-803 and BGC-823 Cells

作　　者：李丹[1] 金凤[1] 陶丽[1] 倪腾洋 王海波[1] 冯俊[1] 朱光 钱亚云[1] 丁岩冰[2] Masataka Sunagawa 刘延庆[1,2] LI Dan;JIN Feng;TAO Li;NI Teng-yang;WANG Hai-bo;FENG Jun;ZHU Guang;QIAN Ya-yun;DING Yan-bing;Masataka Sunagawa;LIU Yan-qing(Cancer Research Institute,Medical College of Integrated Traditional Chinese and Western Medicine,Yangzhou University,Key Laboratory of Syndrome Differentiation and Treatment of Gastric Cancer of State Administration of Traditional Chinese Medicine,Medical ＆ Pharmaceutical Institute of Yangzhou University,Yangzhou 225009,China;Yangzhou First People＇s Hospital,Affiliated Hospital of Yangzhou University,Yangzhou 225000,China;School of Medicine,Showa University,Tokyo 142,Japan)

机构地区：[1]扬州大学医学院中西医结合临床医学系肿瘤研究所、国家中医药管理局胃癌毒邪论治重点研究室、医药研究所,扬州225009 [2]扬州大学附属医院、扬州市第一人民医院,扬州225000 [3]昭和大学医学部,东京142

出　　处：《中国实验方剂学杂志》2018年第19期117-123,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81773944);江苏省自然科学基金项目(BK20141280)

摘　　要：目的：探讨金龙胶囊对人胃癌MGC-803,BGC-823细胞侵袭和迁移能力的影响并对其具体机制进行探讨。方法：体外培养胃癌MGC-803,BGC-823细胞,分为金龙胶囊组（0.1,0.2,0.4,0.8 g·L-1）,空白组和5-氟尿嘧啶（5-FU）组,采用噻唑蓝（MTT）比色法分别检测金龙胶囊对MGC-803,BGC-823细胞增殖的抑制作用,计算半数抑制率（IC50）;采用细胞侵袭（transwell）小室法和划痕实验检测金龙胶囊处理MGC-803和BGC-823细胞24 h后,细胞侵袭和迁移能力发生改变;蛋白免疫印迹法（Western blot）检测金龙胶囊处理MGC-803,BGC-823细胞24 h后E-钙黏素（E-cadherin）,基质金属蛋白酶-2（MMP-2）和基质金属蛋白酶-9（MMP-9）蛋白表达情况。结果：金龙胶囊能明显抑制MGC-803,BGC-823细胞的增殖,呈一定的浓度及时间依赖性;与空白组比较,金龙胶囊能明细减少MGC-803,BGC-823细胞的穿膜细胞数目,可明显影响2种胃癌细胞的侵袭与迁移能力,并呈明显浓度依赖性（P〈0.05）;金龙胶囊组的划痕距离较空白组明显降低,影响MGC-803,BGC-823细胞迁移,并呈浓度依赖性（P〈0.05）;与空白组比较,金龙胶囊组能够提高MGC-803和BGC-823细胞中E-cadherin蛋白表达,降低MMP-2,MMP-9蛋白表达水平,且呈一定的量效关系（P〈0.05）。结论：金龙胶囊能够抑制人胃癌MGC-803,BGC-823细胞的侵袭和迁移能力,其作用机制可能与上调E-cadherin表达,抑制MMP-2,MMP-9表达水平有关。本研究证实了金龙胶囊对胃癌MGC-803,BGC-823细胞抗侵袭转移的部分机制。Objective： To investigate the effect of Jinlong capsule（ JLC） on the invasion and migration of human gastric cancer MGC-803 and BGC-823 cells and its specific mechanism. Method： Gastric cancer MGC-803 and BGC-823 cells were cultured in vitro and divided into JLC group（ with concentrations of 0. 1,0. 2,0. 4,0. 8 g·L-1）,negative control group and 5-fluorouracil（ 5 FU） as positive control group. Methye thiazolye telrazlium（ MTT） was used to detect the inhibitory effect of Jinlong capsule on the proliferation inhibition rate of MGC-803 and BGC-823 cells,in order to calculate the 50% inhibitory concentration（ IC50）; transwell and wound healing assay were used to detect the invasion and migration abilities of BGC-823 cell and MGC-803 cells after treatment with JLC for 24 h; after 24 h,the migration of MGC-803 and BGC-823 cells changed; Western blot method was used to detect the expression levels of E-cadherin,matrix metalloproteinase（ MMP）-2 and MMP-9 after Jinlong capsule treatment for 24 h. Result： JLC could obviously inhibit the proliferation of MGC-803 and BGC-823 cells in a time-dependent manner; compared with the control group,JLC could significantly affect the invasion and migration abilities of the two kinds of gastric cancer cells in a concentration-dependent manner（ P 〈0. 05）; the scratch distance of Jinlong capsule group was significantly lower than that in control group in a concentrationdependent manner（ P〈 0. 05）; compared with control group,JLC group could increase the expression of Ecadherin and reduce the expression levels of MMP-2,MMP-9 in MGC-803 and BGC-823 cells,with a doseresponse relationship（ P〈 0. 05）. Conclusion： JLC can inhibit the invasion and migration of human gastric cancer MGC-803 and BGC-823 cells,and its mechanism may be related to the up-regulation of the expression of Ecadherin and the inhibition of the expression levels of MMP-2, MMP-9. This study has confirmed part of mechanisms of JLC on the invasion and metastasis of gastric canc

关 键 词：金龙胶囊 侵袭 迁移 基质金属蛋白酶-2 基质金属蛋白酶-9 上皮细胞间质转化态 

分 类 号：R22[医药卫生—中医基础理论] R242[医药卫生—中医学]