Single-cell RNA-seq reveals the diversity of trophoblast subtypes and patterns of differentiation in the human placenta  

作　　者：Yawei Liu Xiaoying Fan Rui Wang Xiaoyin Lu Yan-Li Dang Huiying Wang Hai-Yan Lin Cheng Zhu Hao Ge James C. Cross Hongmei Wang 

机构地区：[1]State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 100101 Beijing, China [2]Biomedical Pioneering Innovation Center, College of Life Science, Peking University, 100871 Beijing, China [3]Department of Experimental Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA [4]Department of Obstetrics and Gynecology, The 306th Hospital of PLA, 100101 Beijing, China [5]Department of Obstetrics and Gynecology, Beijing Shijitan Hospital, 100038 Beijing, China [6]Departments of Biochemistry and Molecular Biology, Comparative Biology and Experimental Medicine, Obstetrics and Gynecology, and Medical Genetics, University of Calgary, Calgary, AB T2N 4NI, Canada

出　　处：《Cell Research》2018年第8期819-832,共14页细胞研究（英文版）

基　　金：We thank FT for providing the single-cell RNA-seq and bioinformatics platform. This research was supported by the ＂Organ Reconstruction and Manufacturing＂ Strategic Priority Research Program of the Chinese Academy of Sciences （XDA16020700） and grants from the National Natural Science Foundation of China （81490741, 81322008 and 31501102） and the Ministry of Science and Technology of China （2016YFC1000208）.

摘　　要：The placenta is crucial for a successful pregnancy and the health of both the fetus and the pregnant woman. However, how the human trophoblast lineage is regulated, including the categorization of the placental cell subtypes is poorly understood. Here we performed single-cell RNA sequencing （RNA-seq） on sorted placental cells from first- and second-trimester human placentas. New subtypes of cells of the known cytotrophoblast cells （CTBs）, extravillous trophoblast cells （EVTs）, Hofbauer cells, and mesenchymal stromal cells were identified and cell-type-specific gene signatures were defined. Functionally, this study revealed many previously unknown functions of the human placenta. Notably, 102 polypeptide hormone genes were found to be expressed by various subtypes of placental cells, which suggests a complex and significant role of these hormones in regulating fetal growth and adaptations of maternal physiology to pregnancy. These results document human placental trophoblast differentiation at single- cell resolution and thus advance our understanding of human placentation during the early stage of pregnancy.

关 键 词：BIOINFORMATICS Developmental biology TRANSCRIPTOMICS 

分 类 号：Q132[生物学—普通生物学] TP311[自动化与计算机技术—计算机软件与理论]