广东口岸结核分枝杆菌复合群分子流行病学特征分析  

Molecular epidemiologic characteristics of Mycobacterium tuberculosis complex at Guangdong port

作　　者：吴培丰 温颖[1] 陈嘉 高颖龙 白静[1] 王向阳[1] 吴健[1] WU Pei-feng;WEN Ying;CHEN Jia;GAO Ying-long;BAI Jing;WANG Xiang-yang;WU Jian(Testing Laboratory of Tuberculosis,Guangdong International Travel Healthcare Center,Guangzhou 510635,China)

机构地区：[1]广东国际旅行卫生保健中心结核病检测实验室,广州510635

出　　处：《中国防痨杂志》2018年第9期973-978,共6页Chinese Journal of Antituberculosis

基　　金：国家质量监督检验检疫总局科技计划项目（2016IK061）;广东出入境检验检疫局科技计划项目（2017GDK54）

摘　　要：目的了解广东口岸结核分枝杆菌复合群（MTBC）主要流行菌株及输人性菌株的分子流行病学特征。方法采用熔解曲线间隔区寡核苷酸分型（melting curve spoligotyping，McSpoligotyping）技术对广东口岸558株MTBC菌株进行基因分型，将分型结果与SITVITWEB数据库进行比对，获取间隔区寡核苷酸国际型别（spolig-otype internationaltype，SIT）编号，并用BioNumerics7．6进行聚类分析。结果558株MTBC菌株共分为107种基因型，其中66种归属于8种基因家族及4种家系。家系2和家系4共占菌株总数的86．38％（482／558）。6个主要流行簇分别为SITl、SIT53、SIT52、SIT50、SITl90和SITl9，其中SITl为最大流行簇。17株输人性MTBC包含北京、拉丁美洲和地中海（Latin American and Mediterranean，LAM）、East African Indian（EAI）、T、Africanum（AFRI）及未定义的基因家族，其中包含23．53％（4／17）的新基因型。北京家族菌株耐药率为27．25％（106／389），非北京家族菌株耐药率为23．81％（40／168），差异无统计学意义（X^2=0．72，P=0．40）。典型北京家族菌株耐药率为26．03％（95／365），非典型北京家族菌株耐药率为45．83％（11／24），差异有统计学意义（X^2=4．46，P=0．03）。结论广东口岸以家系2和家系4MTBC为主要流行菌株，输入性菌株以来源地区流行的基因家族和新基因型为主，应加强对主要流行菌株及输人性菌株的分子流行病学监测。非典型北京家族菌株与耐药性具有相关性。Objective To study the molecular epidemiologic characteristics of the main epidemic strains and the imported strains of M. tuberculosis complex （MTBC） at Guangdong port. Methods Five hundred and fifty-eight MTBC strains preserved at Guangdong port were genotyped by melting curve spoligotyping （McSpoligo- typing）. The spoligotype patterns were compared with SITVITWEB database to obtain spoligotype international type （SIT） number and clustered by BioNumerics 7.6 version. Results Five hundred and fifty-eight MTBC strains were divided into 107 spoligotype patterns, among which 66 patterns belonged to 8 families and 4 lineages. Lineage 2 and lineage 4 were accounted for 86.38% （482/558） of the total strains. SIT1, SIT53, SIT52, SIT50, SIT190 and SIT19 were the six main epidemic clusters, among which SIT1 was the biggest one. The 17 imported MTBC contained Beijing, Latin American and Mediterranean （LAM）, East African Indian （EAI）, T, Africanum （AFRI） as well as undefined gene family, including 23. 53% （4/17） new genotypes. No significant difference was observed between Beijing family strains （27. 25%, 106/389） and non-Beijing family strains （23.81~, 40/168） in drug resistanee （X^2=0. 72, P= 0. 40）. Nevertheless, the drug resistant rate of atypical Beijing family strains （45.83%, 11/24） was higher than that of typical Beijing family strains （26.03%, 95/365）, and the differences were significant （X^2= 4. 46, P=0. 03）. Conclusion Lineage 2 and lineage 4 of MTBC are the main epidemic strains at Guangdong port. The imported strains are dominated by gene families prevalent in the source areas and new genotypes. It＇s necessary to strengthen the molecular epidemiologic surveillance of the main epidemic strains and the imported strains. There is a correlation between atypical Beijing family strains and drug resistance.

关 键 词：分枝杆菌 结核 寡核苷酸序列分析 细菌分型技术 基因测定 抗药性 细菌 广东口岸 

分 类 号：R378.911[医药卫生—病原生物学]