奶酪中甲醛含量静态顶空气相色谱测定法  

Determination of formaldehyde in cheese by headspace gas chromatography

作　　者：赵丹莹[1] 温雅[1] 王玮[1] 郭蒙京[1] 李堃[1] ZHAO Dan-ying;WEN Ya;WANG Wei;GUO Meng-jing;LI Kun(Physicochemical Laboratory,Xicheng District Center for Disease Control and Prevention,Beijing,lO0120,China)

机构地区：[1]北京市西城区疾病预防控制中心理化检验科,北京100120

出　　处：《职业与健康》2018年第18期2495-2497,共3页Occupation and Health

基　　金：北京市西城区优秀人才培养资助项目(20160049)

摘　　要：目的建立静态顶空气相色谱法测定奶酪中甲醛含量的新方法。方法取一定量奶酪样品,加入以还原剂0.1 mol/L硼氢化钠和1 mol/L氢氧化钠1∶10比例配制的反应液,使样品内甲醛还原为甲醇,50℃水浴、涡旋提取后直接进行顶空气相色谱FID测定,以外标法定量。结果在1.0～20.0 mg/L线性范围内,回归方程相关系数大于0.998,加标回收率在89.2%～110.8%之间,按照取样量2.0 g,定容体积5 ml计算,方法检出限为0.75 mg/kg,相对标准偏差(RSD)小于8.3%。结论本方法操作简便、快速、准确和稳定,适用于奶酪中甲醛含量的测定。[Objective]To establish a new method for the determination of formaldehyde content in cheese by headspace gas chromatography with reducing agent.[Methods] A certain amount of samples were taken to add reactive solution mixed by 0.1 mol/L sodium borohydride reducing agent and 1 mol/L Na OH with the proportion of 1 ∶10. Formaldehyde in the sample was reduced to methanol,and determined by headspace gas chromatography with FID detector after 50 ℃ water bath and the vortex. External standard method was used for quantitative determination of formaldehyde. [Results] The coefficient of the linear regression equation was higher than 0.998 within the linear range of 1.0-20.0 mg/L,and the recovery rate was 89.2%-110.8%. The detection limit was 0.75 mg/kg and the relative standard deviation（RSD） was less than 8.3%,according to the sampling volume of 2.0 g and the constant volume of 5 ml. [Conclusion] The method is simple,rapid,accurate and stable,and is suitable for the determination of formaldehyde in cheese.

关 键 词：甲醛 甲醇 氧化-还原反应 静态顶空气相色谱 奶酪 

分 类 号：R115[医药卫生—公共卫生与预防医学]