Chemical characterization of fine and ultrafine PM, direct and indirect genotoxicity of PM and their organic extracts on pulmonary cells  

作　　者：Sylvain Billet Yann Landkocz Perrine J. Martin Anthony Verdin Frederic Ledoux Capucine Lepers Veronique Andre Fabrice Cazier Francois Sichel Pirouz Shirali Pierre Gosset Dominique Courcot 

机构地区：[1]University of the Littoral Opal Coast, Unit of Environmental Chmistry and Interactions with Liuing Organisms, UCEIV EA4492, SFR Condorcet FR CNRS 3417, Dunkerque, France [2]Normandy Uniu, UNICAEN, ABTE EA4651, Caen, France [3]University of the Littoral Opal Coast, Common Centre of Measurements, CCM, Dunkerque, France [4]Centre Francois Baclesse, Caen, France [5]Department of Anatomy and Pathological Cytologly, Saint-Vincent Hospital, Catholic Hospital, Lille, France

出　　处：《Journal of Environmental Sciences》2018年第9期168-178,共11页环境科学学报（英文版）

基　　金：supported by the Institut National du Cancer (INCa; Convention no. 2010-368);the Hauts-de-France Region (Convention No. 14003399);the French Agency of the Environment and Energy (ADEME; Convention no. 1494c008283-84)

摘　　要：Particulate matter in ambient air constitutes a complex mixture of fine and ultrafine particles composed of various chemical compounds including metals, ions, and organics. A multidisciplinary approach was developed by studying physico-chemical characteristics and mechanisms involved in the toxicity of particulate atmospheric pollution. PM0.3-2.s and PM2.5 including ultrafine particles were sampled in Dunkerque, a French industrialized seaside city. PM samples were characterized from a chemical and toxicological point of view. Physico-chemical characterization evidenced that PM2.5 comes from several sources： natural ones, such as soil resuspension and marine sea-salt emissions, as well as anthropogenic ones, such as shipping traffic, road traffic, and industrial activities. Human BEAS-2B tung cells were exposed to PM0.3-2.5, or to the Extractable Organic Matter （EOM） of PM0.3-2.5 and PM2.s. These exposures induced several mechanisms of action implied in the genotoxicity, such as oxidative DNA adducts and DNA Damage Response. The toxicity of PM-EOM was higher for the sample including the ultrafine fraction （PM2.5） containing also higher concentrations of polycyclic aromatic hydrocarbons. These results evidenced the major role of organic compounds in the toxicity of PM.Particulate matter in ambient air constitutes a complex mixture of fine and ultrafine particles composed of various chemical compounds including metals, ions, and organics. A multidisciplinary approach was developed by studying physico-chemical characteristics and mechanisms involved in the toxicity of particulate atmospheric pollution. PM0.3-2.s and PM2.5 including ultrafine particles were sampled in Dunkerque, a French industrialized seaside city. PM samples were characterized from a chemical and toxicological point of view. Physico-chemical characterization evidenced that PM2.5 comes from several sources： natural ones, such as soil resuspension and marine sea-salt emissions, as well as anthropogenic ones, such as shipping traffic, road traffic, and industrial activities. Human BEAS-2B tung cells were exposed to PM0.3-2.5, or to the Extractable Organic Matter （EOM） of PM0.3-2.5 and PM2.s. These exposures induced several mechanisms of action implied in the genotoxicity, such as oxidative DNA adducts and DNA Damage Response. The toxicity of PM-EOM was higher for the sample including the ultrafine fraction （PM2.5） containing also higher concentrations of polycyclic aromatic hydrocarbons. These results evidenced the major role of organic compounds in the toxicity of PM.

关 键 词：Air pollution particulate matter PM2.5 Ultrafine particles Physico-chemical characterization BIOTRANSFORMATION GENOTOXICITY 

分 类 号：X51[环境科学与工程—环境工程] X171.5