TRIM59、Akt及MMP-9在肝癌组织中的表达及其临床意义  

Expression of TRIM59、Akt and MMP-9 in hepatocellular carcinoma and correlation with clinical pathological features

作　　者：夏修良[1] 薛栋[1] 相亭海[1] 宋德坤[1] 王建强[1] 李新军[2] XIA Xiu-liang1, XUE Dong1, XIANG Ting-hai1, SONG De-kun1, WANG Jian-qiang1, LI Xin-jun2(1Department of General Surgery Oone,2Department of Pathology, the People ＇s Hospital of Binzhou（Binzhou 256610, China）)

机构地区：[1]山东省滨州市人民医院普外一科,山东滨州256610 [2]山东省滨州市人民医院病理科,山东滨州256610

出　　处：《中国现代普通外科进展》2018年第8期599-602,共4页Chinese Journal of Current Advances in General Surgery

基　　金：山东省科技发展计划项目(2013YD18032)

摘　　要：目的 :探讨三结构域蛋白59(TRIM59)、蛋白激酶B(Akt)和基质金属蛋白酶-9(MMP-9)在肝细胞肝癌中的表达相关性及预后评价。方法:应用免疫组织化学方法回顾性分析TRIM59、Akt及MMP-9蛋白在98例肝癌组织中的表达状况,并分析各指标与临床病理参数之间的关系,应用Spearman等级检验分析TRIM59与Akt、MMP-9的相关性。结果:肝癌组织中TRIM59、Akt及MMP-9蛋白阳性表达率分别是73.5%、65.3%、61.2%,均明显高于癌旁肝组织(P<0.05)。研究发现TRIM59、Akt及MMP-9蛋白表达与病理分级、包膜完整、肿瘤分化、血管侵犯和TNM分期明显相关(P均<0.05)。肝癌组织中TRIM59与Akt、MMP-9表达均呈正相关(P<0.05),Akt和MMP-9表达也呈正相关(P<0.05)。生存分析表明,TRIM59可作为肝癌预后的一个独立性因素。结论:TRIM59、Akt和MMP-9在肝癌组织中明显高表达。TRIM59过表达与肝癌恶性进展和患者预后密切相关,提示TRIM59是肝癌患者的潜在治疗靶点。Objective： To explore the potential correlation and prognostic value of TRIM59, Akt and MMP-9 expression in hepatocellular carcinoma （HCC）. Methods： We retrospectively exam- ined the simultaneous expression of TRIM59, Akt and MMP-9 with immunohistochemistry in the paraffin blocks of 98 patients who underwent radical resection. The Spearman method was used to explore the correlation between TRIM59, Akt and MMP-9 in HCC tissues. Results： TRIM59, Akt and MMP-9 showed a high expression level of 73.5%,65.3% and 61.2% in HCC, respectively. The positive expression rate of TRIM59, Akt and MMP-9 protein in hepatocellular carcinoma group were all higher than those in adjacent paracancerous tissues group （P 〈 0.05）. Additionally, our results showed that TRIM59, Akt and M M P-9 protein expression in H CC tissues was significantly associat- ed with pathological grade（P〈0.05）,invelope integrity（P〈0.05）, differentiation（P〈0.05）, vascular inva- sion（P〈0.05） and TNM stage（P〈0.05）. Significantly positive correlations was found between TRIM59 and Akt, MMP-9 by using spearman correlation analysis（all P〈0.05）. There was positive correlation between Akt and MMP-9 （P〈0.05）. Moreover, patients＇ survival was negatively correlated with TRIM59 protein expression. Furthermore, we found that TRIM59 protein was an independent prog- nostic factor in hepatocellular carcinoma. Conclusion： Our data showed that TRIM59, Akt and MMP-9 expressions were significantly elevated in HCC tissues. The overexpression of TRIM59 is closely associated with HCC progression and poor survival of the patients, indicating TRIM59 is a potential therapeutic target for HCC patients.

关 键 词：肝细胞癌 三结构域蛋白59 蛋白激酶B 基质金属蛋白酶-9 免疫组织化学 

分 类 号：R735.7[医药卫生—肿瘤]