D-双功能蛋白在肝癌组织中的表达及其与AFP和CA19-9的相关性  

Expression of D-bifunctional protein in hepatocellular carcinoma tissue and its correlation with alpha-fetoprotein and carbohydrate antigen 19-9

作　　者：路欣 孔令玉[2] 刘殿卿[1] 许林骥 邱宁 程华[3] LU Xin, KONG Lingyu, LIU Dianqing, et al.(Department of Clinical Laboratory, Tangshan Maternal and Child Health Hospital, Tangshan, Hebei 063000, China)

机构地区：[1]唐山市妇幼保健院检验科,河北唐山063000 [2]河北医科大学,石家庄050017 [3]唐山市妇幼保健院病理科,河北唐山063000

出　　处：《临床肝胆病杂志》2018年第10期2153-2156,共4页Journal of Clinical Hepatology

基　　金：河北省2018年医学科学研究重点课题计划(20181317)

摘　　要：目的探讨D-双功能蛋白(DBP)在肝细胞癌(HCC)组织中的表达情况,以及与肿瘤血清学标志物AFP、CA19-9表达的相关性,进而分析DBP对HCC早期诊断和预后判断的临床价值。方法选择唐山市妇幼保健院和唐山市人民院2015年6月-2017年5月行肝癌切除术并随访的20例患者作为试验组,同期两所医院的20例健康体检者作为对照组,应用化学发光法检测AFP、CA19-9的表达水平,免疫组化法检测肝癌组织和癌旁组织DBP的表达水平,用积分光密度值(IOD)进行测量。计量资料2组间比较采用t检验,检测指标相关性检验采用Pearson相关分析。结果肝癌组织DBP的IOD值(220. 52±101. 30)明显高于癌旁组织(40. 49±19. 32),差异具有统计学意义(t=-7.00,P <0.01)。且DBP与AFP(r2=0. 868,P <0. 01)和CA19-9(r2=0. 814,P <0. 01)的表达水平均呈正相关。结论 HCC患者DBP表达升高,与HCC的发生发展密切相关,为肝癌的诊断提供新的靶点。Objective To investigate the expression of D-bifunctional protein（ DBP） in hepatocellular carcinoma（ HCC） tissue and its correlation with serum tumor markers alpha-fetoprotein（ AFP） and carbohydrate antigen 19-9（ CA19-9）,as well as the clinical value of DBP in early diagnosis and prognostic evaluation of HCC. Methods A total of 20 patients who underwent hepatectomy for liver cancer in Tangshan Maternal and Child Health Hospital and Tangshan People＇s Hospital from June 2015 to May 2017 were enrolled as experimental group,and 20 healthy subjects who underwent physical examination in these two hospital during the same period of time were enrolled as control group. Chemiluminescence was used to measure the expression of AFP and CA19-9,and immunohistochemistry was used to measure the expression of DBP,which was expressed as integrated optical density（ IOD）,in HCC tissue and adjacent tissue. The t-test was used for comparison of continuous data between groups,and the Pearson correlation analysis was used to investigate correlation between indices. Results The IOD value of DBP in HCC tissue was significantly higher than that in adjacent tissues（ 220. 52 ± 101. 30 vs 40. 49 ±19. 32,t =-7. 00,P〈0. 01）. The expression of DBP was positively correlated with that of AFP（ r^2= 0. 868,P〈0. 01） and CA19-9（ r^2= 0. 814,P〈0. 01）. Conclusion The expression of DBP increases in HCC patients,which is closely associated with the development and progression of HCC. Therefore,it provides a new target for the diagnosis of HCC.

关 键 词：癌 肝细胞 雌二醇脱氢酶类 甲胎蛋白类 CA-19-9抗原 

分 类 号：R735.7[医药卫生—肿瘤]