伴微乳头结构的肺腺癌组织中EGFR及KRAS的表达变化及意义  

Changes and significance of the expression of EGFR and KRAS in lung adenocarcinoma with micro papillary structure

作　　者：邱娟 QIU Juan(Department of Pathology,Henan Jiaozuo Coal（Group）Limited Liability Company Central Hospital,Jiaozuo,Henan 454000,China)

机构地区：[1]河南省焦作煤业(集团)有限责任公司中央医院病理科,河南焦作454000

出　　处：《临床肺科杂志》2018年第11期2000-2003,共4页Journal of Clinical Pulmonary Medicine

摘　　要：目的探讨表皮生长因子受体(EGFR)、鼠Kirsten肉瘤病毒致癌基因(KRAS)表达与伴微乳头结构的肺腺癌患者临床病理参数间的关系。方法采用SP免疫组织化学染色方法检测伴微乳头结构的肺腺癌组织及对应癌旁正常组织中EGFR及KRAS蛋白的表达,运用Western blot法检测伴微乳头结构的肺腺癌组织及对应癌旁正常组织中EGFR及KRAS蛋白的表达量,并分析EGFR及KRAS蛋白阳性表达情况与患者临床病理特征的关系,采用Spearman等级相关分析EGFR及KRAS在伴微乳头结构的肺腺癌组织中表达的相关性。结果伴微乳头结构的肺腺癌组织中的EGFR与KRAS蛋白表达阳性率分别为74. 4%(67/90)、83. 3%(75/90),明显高于癌旁正常组织的15. 2%(7/46)、19. 6%(9/46)(P=0. 025,0. 018)。癌组织中EGFR、KRAS蛋白表达水平分别为0. 7136±0. 0652、0. 8751±0. 0347,明显高于癌旁正常组织的0. 2148±0. 0124、0. 2308±0. 0136 (P=0. 032,0. 029)。EGFR蛋白阳性表达率与TNM分期、淋巴结转移有关(P=0. 022,0. 015),KRAS蛋白阳性表达率与TNM分期、分化程度、淋巴结转移有关(P=0. 017,0. 024,0. 011)。经Spearman相关分析显示,癌组织中EGFR与KRAS表达呈正相关(r=0. 516,P=0. 023)。结论 EGFR、KRAS蛋白表达在伴微乳头结构的肺腺癌组织中明显上调,且两者呈正相关的关系,在伴微乳头结构的肺腺癌发生及发展中具有重要作用。EGFR、KRAS检测可以作为早期诊断伴微乳头结构的肺腺癌的生物学指标。Objective To investigate the relationship belween the expression of epidermal growth factor re- ceptor （EGFR） and Kirsten sarcoma virus oncogene （KRAS） and clinicopathological parameters in patients with lung adenocarcinoma with micro papillary structure. Methods The expression of EGFR and KRAS protein in lung adenocarcinoma tissues and corresponding micro papillary carcinoma of adjacent normal tissues was detected by SP immunohistochemistry, and the expression level of EGFR and KRAS protein was detected by Western blot assay. The relationship between the expression of EGFR and KRAS protein and clinicopathological features was analyzed by Spearman grade. Results The positive rate of EGFR and KRAS protein expression in lung adenocarcinoma tissues with micro papilla structure were 74.4% （ 67/90 ） and 83.3 % （ 75/90 ）, respectively, which were significantly higher than those in adjacent normal tissues （ 15.2% （7/46） and 19.6% （9/46） （P =0. 025, 0. 018）. The expression levels of EGFR and KRAS in cancer tissues were 0. 7136 ±0. 0652 and 0. 8751 ±0. 0347, which were significantly higher than those in adjacent normal tissues, which were 0. 2148 ±0. 0124 and 0. 2308 ±0. 0136 （P =0. 032, 0. 029）. The positive expression rate of EGFR protein was related to TNM stage and lymph node metastasis （P = 0. 022, 0. 015）. The positive expression rate of KRAS protein was related to TNM stage, differentiation degree and lymph node metastasis （P = 0. 017, 0. 024, 0. 011 ）. Spearman correlation analysis showed that the expression of EGFR in cancer tissues was positively correlated with the expression of KRAS （r = 0. 516, P = 0. 023）. Conclusion The expression of EGFR and KRAS protein is significantly up-regulated in lung adenocarcinoma tissues with micro papillary structure, and has a positive correlation with them. It plays an important role in the occurrence and development of lung adenocarcinoma with micro papilla structure. The detection of EGFR and KRAS can be used as a biological

关 键 词：表皮生长因子受体 鼠Kirsten肉瘤病毒致癌基因 伴微乳头结构的肺腺癌 免疫组织化学 

分 类 号：R734.2[医药卫生—肿瘤]