全自动DNA定量分析在肺泡灌洗液中的临床应用价值  

Clinical value of automatic DNA image cytometry in bronchoalveolar lavage fluid

作　　者：石安琪 王敏[1] 向莱 江涛[1] SHI An-qi;FANG Min;XIANG Lai;JIANG Tao(Department of Respiratory and Critical Care Medicine,the First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China)

机构地区：[1]重庆医科大学附属第一医院呼吸与危重症医学科,重庆400016

出　　处：《临床肺科杂志》2018年第11期2024-2027,共4页Journal of Clinical Pulmonary Medicine

基　　金：重庆市科委应用开发项目(No cstc2014yykfA110026)

摘　　要：目的探讨全自动DNA定量分析在肺泡灌洗液中的应用价值。方法收集合格肺泡灌洗液标本386例,同时送检液基细胞学检查及全自动DNA定量分析检查,并随访病检结果、肺癌病例分期结果。结果共168例有明确病理学证据诊断为肺癌,其中52例未发现异倍体细胞; 218例标本未找到明确病理学肿瘤证据,其中43例找到异倍体细胞。DNA-ICM与病理学对肺泡灌洗液的诊断价值差异具有统计学差异(P<0. 05)。非小细胞肺癌病例中,细胞DNA含量与肿瘤分期呈正相关,相关系数为3. 28,P <0. 001;与肿瘤部位、肿瘤类型无明显相关性。结论 DNA-ICM检查对肺癌的诊断价值不及病理学检查,但细胞DNA含量与肿瘤分期呈正相关,可协助评估肿瘤预后。Objective To explore the application value of automatic DNA image cytometry in bronchoalveolar lavage fluid. Methods 386 samples of bronehoalveolar lavage fluid were collected, and liquid based cytology test and automatic DNA image eytometry exanfination were performed simultaneously. The pathological exanfination results and staging of lung cancer were followed-up. Results A total of 168 eases were diagnosed as lung cancer with definite pathological evidence, of which 52 eases were not found aneuploid cells, and 218 eases had no definite pathological tumor evidence, among which 43 eases were found aneuploid cells. There were significant differences in the diagnostic value of DNA-ICM and pathology in bronchoalveolar lavage fluid （ P 〈 0. 05 ）. In non-small cell lung cancer cases, DNA content was positively correlated with tumor stage, the correlation coefficient was 3.28, P 〈 0. 001, and there was no significant correlation with tumor location and tumor type. Conclusion The diagnostic value of DNA- ICM for lung cancer is less than that of pathological exanfination, but the content of DNA is positively correlated with the stage of tumor, which can help to evaluate the prognosis of the tumor.

关 键 词：肺癌 DNA定量分析 肺泡灌洗液 

分 类 号：R734.2[医药卫生—肿瘤] R730.43[医药卫生—临床医学]