双偶氮染料的茶渣固态漆酶脱色  

Decolorization of disazo dyes with solid laccase on tea residue

作　　者：孙珂 马安周[2] 马海乐[1] 丁重阳 王锋[1] SUN Ke1, MA An-zhou2, MA Hai-le1, DING Zhong-yang3, WANG Feng1(1.School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China; 2.Research Center for Eco - Environmental Sciences, Chinese Academy of Sciences, Beifing 100085, China; 3.National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China)

机构地区：[1]江苏大学食品与生物工程学院,江苏镇江212013 [2]中国科学院生态环境研究中心,北京100085 [3]江南大学粮食发酵工艺与技术国家工程实验室,江苏无锡214122

出　　处：《印染》2018年第18期17-23,共7页China Dyeing and Finishing

基　　金：江苏省自然科学基金(BK20160493);中国博士后科学基金(2015M571691);江苏大学高级人才科研启动基金(15JDG17和JDG061);江苏大学2014年度校"青年骨干教师培养工程"项目

摘　　要：利用变色栓菌茶渣固态发酵制备的茶渣固态漆酶对双偶氮类染料进行脱色,并以直接耐晒黄RS为代表,研究其脱色机制和脱色效果。结果表明,在茶渣固态漆酶用量为160 U/L、染料质量浓度为100 mg/L条件下反应2 h后,直接耐晒黄RS的脱色率达到81.10%。研究发现,茶渣固态漆酶的脱色机制包括游离漆酶降解、茶渣吸附和固定化漆酶降解三部分,对直接耐晒黄RS的脱色率分别为16.40%、43.20%和19.70%。茶渣固态漆酶在染料脱色中表现出较好的重复使用性能,重复利用4次,脱色率仍保持在50%以上。毒性检测结果表明,绿豆种子的萌发率从36.67%提高到85%,脱色后染料溶液毒性明显下降。利用茶渣固态漆酶对染料脱色具有时间短、效率高、可重复利用和成本低等优势,在染料脱色方面具有良好的前景。Solid laccase on tea residue is prepared from the laccase produced from Trametes versicolor by solid-state fermentation. The solid laccase is used for the decolorization of disazo dyes. Decolorization mechanism and efficiency are evaluated using Direct Fast Yellow RS as the representative example. The optimal dosage of solid laccase is 160 U/L with respect to the dye concentration of 100 mg/L. After 2 h incubation, the decolorization efficiency of Direct Fast Yellow RS reaches 81.10%. It indicates that the decolorization mechanism of solid laccase on tea residue includes three parts： degradation by free laccase, adsorption by tea residue and degradation by immobilized laccase, where the decoloriza- tion efficiencies of Direct Fast Yellow RS are 16.40%, 43.20% and 19.70% respectively. The solid laccase on tea residue exhibits good reusability for the dye decolorization. The decolorization efficiency remains more than 50% after 4 recycles. The toxicity test shows that the germination rate of mung bean seeds increases from 36.67% to 85% after decolorization. It indicates that the toxicity of dye solution decreases significantly after decolorization. These results show that the dye decolorization by solid laccase on tea residue has some advantages, including short reaction time, high efficiency, good reusability and low cost. It has good prospects in dye decolorization and provides a new idea for the comprehensive utilization of tea residue.

关 键 词：废水处理 固态发酵 漆酶 茶渣 双偶氮染料 脱色 

分 类 号：TS199[轻工技术与工程—纺织化学与染整工程]