人体端粒长度分布特点及变化  

Characteristics of distribution and changes of telomere length in human

作　　者：叶兴[1] 贺子强 邓鹏飞[1] 魏毓 周建明[1] 黄河浪[1] YE Xing, HE Ziqiang, DENG Pengfei, WEI Yu, ZHOU Jianming, HUANG Helang(Jiangxi Province Key Laboratory of Preventive Medicine, Nanchang University, Nanchang 330006, Chin)

机构地区：[1]南昌大学江西省预防医学重点实验室,南昌330006

出　　处：《中南大学学报（医学版）》2018年第9期945-949,共5页Journal of Central South University ：Medical Science

基　　金：国家自然科学基金(81360446)~~

摘　　要：目的:通过分析比较不同年龄人群的端粒相对长度,探索端粒长度改变与年龄的关系,为老年医学问题的深入研究提供数据和参考。方法:采用随机抽样的方法,从数家医院健康体检人群中抽取20岁以上者作为研究对象,收集其外周血白细胞作为检测标本。采用实时荧光定量PCR法进行端粒相对长度检测,分析年龄与端粒长度改变的关系。结果:共取样本1 022个,不同年龄组的相对端粒长度的差异有统计学意义(F=21.492,P<0.001),端粒长度与年龄呈负相关(r=–0.325,P<0.001),两者之间的回归方程为:y=–0.008x+1.772(P<0.001)。结论:健康人外周血白细胞端粒长度在各年龄段人群间有差异,且端粒长度随年龄增加逐渐缩短。Objective： To explore the relationship between telomere length changes and age, and to provide data and reference for further study of geriatric medical problems. Methods： Th e healthy people over 20 years old were chosen as subject from several hospitals by random sampling method, and their peripheral blood samples were collected. Th e relative length of telomere was detected by quantitative real-time PCR, and the relationship between age and telomere length was analyzed by statistical soft ware. Results： A total of 1 022 samples were obtained. Th ere were signifi cant diff erences in the relative telomere length among diff erent age groups （F=21.492, P〈0.001）. Telomere length and age showed negative correlation （r=–0.325, P〈0.001）, the regression equation was y=–0.008x＋1.772 （x for age, y for the average telomere length, P〈0.001）. Conclusion： Th e telomere length for peripheral blood leukocytes in healthy people varies between different age groups, suggesting that telomere length gradually decreases with age.

关 键 词：端粒 年龄 性别 实时荧光定量PCR法 老年医学 

分 类 号：R394[医药卫生—医学遗传学]