Stomatin样蛋白2(SLP-2)抑制裸鼠食管鳞状细胞癌移植瘤生长的研究  

Inhibitory effect of stomatin-like protein 2(SLP-2) on growth of transplanted tumor of esophageal squamous cell carcinoma in nude mice

作　　者：宋帅[1] 田地 崔永[1] 高志[1] 王天佑[1] 胡健[1] 常栋[1] SONG Shuai;TIAN Di;CUl Yong;GAO Zhi;WANG Tianyou;HU Jian;CHANG Dong(Department of Thoracic Surgery,Beijing Friendship Hospital,Capital Medical University,Beijing,100050,P.R.China;Department of Infectious Diseases,China Meitan General Hospital,Beijing,100028,P.R.China)

机构地区：[1]首都医科大学附属北京友谊医院胸外科,北京100050 [2]煤炭总医院感染性疾病科,北京100028

出　　处：《中国胸心血管外科临床杂志》2018年第10期885-889,共5页Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

基　　金：北京友谊医院科研启动基金(Z20140512030019)

摘　　要：目的观察stomatin样蛋白2(SLP-2)表达降低后裸鼠体内原位移植肿瘤的生长变化,进一步研究SLP-2在食管鳞状细胞癌(鳞癌)发生、发展中的作用。方法采用RNA干扰技术,建立特异性抑制目的基因SLP-2表达和荧光素酶稳定表达的食管鳞癌细胞株(SLP-2-1质粒感染的细胞、SLP-2-2质粒感染的细胞)。将健康雌性裸鼠(体重19~22 g)随机分为3组,每组12只,6只用于建立皮下移植瘤,另6只用于建立原位移植瘤模型;组1为SLP-2-1质粒感染的细胞,组2为SLP-2-2质粒感染的细胞,组3为空载质粒(SHGFP)感染的细胞。当某一只裸鼠开始出现体重减轻、一般情况差后为实验终点。在处死原位移植瘤裸鼠前利用活体成像技术检测肿瘤的荧光素酶值(ROI);处死裸鼠后,将原发肿瘤送病理学检查。结果组1与组2的ROI值差异无统计学意义(P=0.943);组1和组2的ROI值均明显低于组3(P=0.002、0.000)。组1、组2和组3移植肿瘤均有侵袭浸润至食管肌层的表现。结论 SLP-2参与食管鳞癌的发生、发展过程,降低SLP-2表达可以抑制食管鳞癌的生长。Objective To observe the growth oforthotopic transplanted tumor in nude mice after stomatin-like protein 2 （SLP-2） expression decreased, and to further study the role of SLP-2 in the development and progression of esophageal squamous cell carcinoma. Methods Using RNA interference technique, esophageal squamous cell carcinoma cell lines with specific expression of SLP-2 and stable expression ofluciferase were established. The healthy female nude mice with weight ranging from 19 to 22 g were randomly divided into 3 groups （n=12）, 6 mice were used to establish subcutaneous xenografts, and the other 6 mice were used to establish the orthotopic transplanted tumor model （Group 1： cell infected with SLP-2-1 plasmid; group 2： cell infected with SLP-2-2 plasmid; group 3： cell infected with SHGFP plasmid）. Index of the experiment end was weight loss and poor general situation in any mouse. Before the nude mice were sacrificed, the luciferase value of the tumor was detected by using in vivo imaging technique. After the nude mice were sacrificed, the primary tumor was removed for pathology examination. Results There was no significant difference in region of interest （ROI） value between the group 1 and group 2 （P=0.943）. The ROI value for both groups 1 and 2 was significantly lower than that in the group 3 （P=0.002, P=0.000）. The primary tumor infiltrated into the muscularis propria of esophageal was observed in all groups. Condusion SLP-2 is involved in the development and progression of esophageal squamous cell carcinoma, and the decrease of SLP-2 expression can inhibit the growth of esophageal squamous cell carcinoma.

关 键 词：Stomatin样蛋白2 食管鳞状细胞癌 原位移植动物模型 活体成像 

分 类 号：R735.1[医药卫生—肿瘤]