代谢组学在毒理学研究中的应用  

Application of metabolomics in toxicology

作　　者：卢大胜[1,2] 薛黎明[1] 冯超[1] 金玉娥[1] 张琳 汪国权[1] 周志俊[2] LU Da-sheng;XUE Li-ming;FENG Chao;JIN Yu-e;ZHANG-Lin;WANG Guo-quan;ZHOU Zhi-jun(Division of Chemical Toxicity and Safety Assessment,Shanghai Municipal Center for Disease Control and Prevention,Shanghai 200336,China;School of Public Health,Fudan University,Shanghai 200032,China)

机构地区：[1]上海市疾病预防控制中心化学品毒性鉴定所,上海200336 [2]复旦大学公共卫生学院,上海200032

出　　处：《环境与职业医学》2018年第9期855-860,共6页Journal of Environmental and Occupational Medicine

基　　金：上海市自然科学基金(编号:18ZR1432200);上海市新三年行动毒理学海外高端人才培养(编号:GWTD2015S03);国家自然科学基金(编号:81872643)

摘　　要：代谢组学是继基因组学、转录组学及蛋白质组学之后发展起来的一门新兴组学,是整合包括色谱-质谱(MS)和/或核磁共振(NMR)等现代分析技术、生物化学以及生物信息学等学科的一门交叉学科技术,用于研究生命活动链条下游的代谢物内稳态情况。代谢组学作为传统毒理学毒性研究技术的有效补充,以人体、实验动物和细胞等为对象,通过分析生物流体、细胞和组织样品受内、外因素干预后代谢物种类及数量的变化,进行快速准确的早期毒性筛选以及后期毒性机理研究和毒性生物标志物的发现。本文拟从代谢组学的技术发展以及其在毒理学中的应用进展和存在的问题等方面进行综述性回顾。Metabolomics has been labeled one of the new ＂omics＂, joining genomics, transcriptomics, and proteomics as an interdisciplinary science integrating many advanced analytical technologies including chromatography-mass spectrometry （MS） and/or nuclear magnetic resonance （NMR）, biochemistry, and bioinformatics. Metabolomics, as a tool of understanding global systems biology, has been employed to investigate the homeostasis of downstream metabolites generated by biological activities. Metabolomics is an emerging alternative and complementary technology in toxicology study as it has been widely used to rapidly diagnose early toxicity, explore subsequent toxic mechanism, and identify corresponding biomarkers by profiling metabolite changes induced by toxins and/or toxicants in biofluids, cells, and tissues. This article reviewed and discussed the current technical development of metabolomics, and its applications and challenges in toxicology.

关 键 词：代谢组学 毒理学 代谢通路 质谱 核磁共振 

分 类 号：R994.6[医药卫生—毒理学] X132[医药卫生—药学]