新疆维吾尔自治区伊犁地区女性HPV感染状况及基因型别分布  

Screening different HPV genotypes infection and type-specific in cervical exfoliated cells of women in Yili area of Xinjiang Uygur Autonomous Region, China

作　　者：潘贞贞 宋宇宁 张琴 于娇娇 张克南 梁娜 张娜 马欣 朱君玲[4] 者湘漪 夏哈代提 郑威楠[2] 李洪涛[2] 曹冬冬[2] 潘泽民[2] Pan Zhenzhen;Song Yuning;Zhang Qin;Yu Jiaojiao;Zhang Kenan;Liang Na;Zhang Na;Ma Xin;Zhu Junling;Zhe Xiangyi;Xia Hadaiti;Zheng Weinan;Li Hongtao;Cao Dongdong;Pan Zemin(Department of Clinical Laboratory,the Fourth Division Hospital of Xinjiang Production and Construction Corps,Yili 835000,Chin;Department of Clinical Laboratary,Friendship Hospital of Yili Kazak Autonomous Region of Xinjiang Uygur Autonomous Region,Yili 835000,China)

机构地区：[1]新疆生产建设兵团第四师医院检验科,伊犁835000 [2]新疆维吾尔自治区地方与民族高发病教育部重点实验室/石河子大学医学院生物化学教研室 [3]新描维吾尔自治区伊犁哈萨克自治州友谊医院检验科 [4]新疆维吾尔自治区喀什地区第一人民医院病理科

出　　处：《中华预防医学杂志》2018年第9期946-950,共5页Chinese Journal of Preventive Medicine

基　　金：国家自然科学基金（U1503125、81660462、30860302、30660193）;兵团国际科技合作项目（2013BC003）;石河子大学重大科技攻关计划（gxjs2013-zdgg05）;高层次人才科研启动资金（RCZX201534）

摘　　要：目的 分析新疆维吾尔自治区（新疆）伊犁地区不同民族女性HPV的感染状况及基因型分布情况.方法 采用方便抽样法,选择2015年11月至2017年5月在新疆伊犁地区某综合性医院妇科就诊的54 760名女性为研究对象,采集其宫颈黏液脱落细胞样本,共采集了3 445名;同时收集其民族和年龄等社会信息.纳入标准为有性生活者,宫颈完整者,且民族为汉族、维吾尔族或哈萨克族.采用PCR反向点杂交法,对脱落细胞进行HPV基因分型检测;宫颈癌的诊断由医生根据研究对象的病史、症状、妇科检查结果,以及宫颈细胞学和组织学活检进行确诊.采用χ^2检验比较不同民族研究对象HPV阳性率的差异.结果 研究对象的HPV阳性率为25.6%（882例）;汉族、维吾尔族和哈萨克族HPV阳性率分别为27.9%（564/2 022）、22.9%（196/857）和21.6%（122/566）（χ^2=13.80,P=0.001）.汉族阳性者中感染比例最高的3个亚型依次为HPV16/52/58型,分别占24.8%（140例）、17.7%（100例）和9.8%（55例）;维吾尔族依次为HPV16/52/53型,分别占34.2%（67例）、12.8%（25例）、9.2%（18例）;哈萨克族依次为HPV16/53/52型,分别占37.7%（46例）、17.2%（21例）、12.3%（15例）.维吾尔族≥61岁研究对象HPV阳性率最高,为41.5%（22例）,36-40岁组最低,为15.9%（21例）,差异有统计学意义（χ^2=35.01,P〈0.001）.结论 新疆伊犁地区汉族、维吾尔族和哈萨克族HPV阳性率存在差异,并且感染HPV基因型在不同民族间分布不同.Objective To investigate the infection status and genotype distribution of cervical human papillomavirus （HPV） in women of different ethnic groups and different ages in Yili, Xinjiang Uygur Autonomous Region （Xinjiang）. Methods By using the convenient sampling method, 54 760 women from November 2015 to May 2017 seeking for service in gynecological clinics in a general hospital in Yili, Xinjiang, were selected as the research subjects,and 3 445 samples of cervical mucous exfoliative cells were collected, and the social information of their ethnic and age was collected at the same time. The inclusion criteria were those with sexual life, cervical integrity, and ethnic groups for Han or Uygur or Kazak. PCR-reverse dot blot hybridization was used to detect HPV genotyping in exfoliated cells, and chi-square test was used to compare the difference of HPV positive rate among different ethnic groups. Then, according to ethnicity and age, the differences in positive rates of different ages and ethnic groups were compared in each layer. Results The positive rate of HPV was 25.6% （882 cases）, of which the Han, Uygur and Kazakh were 27.9% （564 cases）, 22.9% （196 cases） and 21.6% （122 cases）, and the difference was statistically significant （χ^2=13.80, P=0.001）. The most prevalent high-risk genotypes of Han women were HPV16/52/58, accounting for 24.8% （140 cases）, 17.7% （100 cases） and 9.8% （55 cases）, respectively. The most prevalent high-risk genotypes of Uygur women were HPV16/52/53, accounting for 34.2% （67 cases）, 12.8% （25 cases）, 9.2% （18 cases）, respectively. The most prevalent high-risk genotypes of Kazak were HPV16/52/53, accounting for 37.7% （46 cases）,17.2% （21 cases）, 12.3% （15 cases）, respectively. The highest rate of HPV in Uygur patients aged≥61 years was 41.5% （22 cases）, and the lowest in group 36-40 years old, 15.9% （21 cases）, the difference between different age groups was statistically significant （χ^2=35.01, P〈0.001）. Conc

关 键 词：子宫颈 癌 基因型 人乳头瘤病毒 

分 类 号：R737.33[医药卫生—肿瘤] R711.3[医药卫生—临床医学]