HPTLC-SERS快速检定葡萄汁中苏丹染料掺假  

Screening of Sudan dyes adulteration in grape juice based on HPTLC-SERS detection

作　　者：王了 叶之阳 王天楠 徐学明[1,2] 陈益胜 杨哪[1,2] 吴凤凤[1,2] 金亚美[1,2] WANG Liao1, YE Zhi-yang1, WANG Tian-nan1,XU Xue-ming1,2,CHEN Yi-sheng1,2,YANG Na1,2, WU Feng-feng1,2, JIN Ya mei1,2(1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi , Jiangsu 214122, China;2. School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, Chin)

机构地区：[1]江南大学食品学院食品科学国家重点实验室,江苏无锡214122 [2]江南大学食品学院,江苏无锡214122

出　　处：《食品与机械》2018年第7期59-64,77,共7页Food and Machinery

基　　金：国家重点研发计划(编号:2017YFF0207800);江苏省自然科学基金(编号:BK20170177);中国博士后基金(编号:2017M610298)

摘　　要：建立并优化一种基于高效薄层色谱和表面增强拉曼联用检测技术的方法,用于快速准确定性定量检测葡萄汁中的苏丹染料掺伪。使用石油醚/丙酮/乙酸(9/1/0.1,体积比)作为流动相在硅胶板上对目标物进行色谱分离,考察纳米银胶体浓度、外加电解质种类、浓度以及入射激光波长等参数对检测信号质量的影响。结果表明,10倍浓缩的纳米银胶体,633nm入射激光,辅以0.5~1.0mol/L NaCl最适合4种目标物的检测。优化检测条件后,检测灵敏度可达0.01~0.05mg/kg;在1~20 mg/kg浓度范围内,检测结果表现出良好的线性关系,R^2>0.996。不仅如此,对葡萄汁样品的测试中,该方法也表现出良好的准确性(添加回收率72%~104%)和精密度(RSD 4.5%~9.6%),因此特别适于大批量饮料和食品样品中非法色素添加的筛检。This paper presented a fast screening method for determining Sudan dye adulteration in grape juice,based on the combination of High Performance Thin-Layer Chromatography（HPTLC）and Surface Enhanced Raman Spectroscopy（SERS）.Chromatography separation was achieved on silica gel plates using petroleum/acetone/acetic acid（9/1/0.1）as the mobile phase.After that,key factors including silver nano particle concentrations,electrolyte types/concentrations and incident laser wavelength was investigated with regarding to their effects on the quality of SERS results.Under optimized conditions,the method sensitivity reached to 0.01 ～0.05 mg/kg,while remarkable linearity（R2〉0.996）was obtained within the range of 1～20 mg/kg.Apart from that,the established method showed satisfactory accuracy（standard-spiking recovery rates 72%～104%）and precision（RSD 4.5%～9.6%）in the validation with the grape juice sample.

关 键 词：表面增强拉曼 高效薄层色谱 热点 苏丹染料 果汁 

分 类 号：O657.37[理学—分析化学] TS255.44[理学—化学]