脊髓CCR5在瑞芬太尼诱发切口痛大鼠痛觉过敏中的作用  

Role of spinal CCR5 in remifentanil-induced hyperalgesia in rats with incisional pain

作　　者：李楠[1] 王新 张麟临 舒瑞辰 郭素倩 赵亓 王国林 Li Nan, Wang Xin, Zhang Linlin, Shu Ruichen, Guo Suqian, Zhao Qi, Wang Guolin,(Department of Anesthesiology, Tianjin Medical University General Hospital Tianjin Research Institute of An- esthesiology, Tianjin 300052, China)

机构地区：[1]天津医科大学总医院麻醉科天津市麻醉学研究所,300052 [2]济宁市第一人民医院麻醉科工作

出　　处：《中华麻醉学杂志》2018年第5期575-578,共4页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（30972847,81371245,81571077）

摘　　要：目的评价脊髓趋化因子cc亚族受体5（CCR5）在瑞芬太尼诱发切口痛大鼠痛觉过敏中的作用。方法取鞘内置管和尾静脉置管成功的雄性sD大鼠32只，体重240～260g，2-3月龄，采用随机数字表法分为4组（n=8）：对照组（C组）、CCR5拮抗剂maraviroc组（M组）、瑞芬太尼＋切口痛组（R＋I组）和maraviroc＋瑞芬太尼＋切口痛组（M＋R＋I组）。C组鞘内注射PBS10μl，尾静脉输注生理盐水1μg·kg^-1·min。60min；M组鞘内注射maraviroc100pmol（溶于10μl PBS中），尾静脉输注生理盐水1μg·kg^-1·min^-160min；R＋I组鞘内注射PBS10txl，然后建立切1：3痛模型，同时尾静脉输注瑞芬太尼1μg·kg^-1·min^-160min；M＋R＋I组鞘内注射maraviroc100pmol（溶于10μl PBS中），然后建立切I：1痛模型，同时尾静脉输注瑞芬太尼1μg·kg^-1·min^-160min。于输注瑞芬太尼或生理盐水前24h（T1）、停止输注瑞芬太尼或生理盐水后2、6、24和48h（T1-4）时测定机械缩足反应阈（MWT）和热缩足潜伏期（TWL），最后1次测定痛阈后处死大鼠，取脊髓L4-6节段，采用Westernblot法测定胶质纤维酸性蛋白（GFAP）和离子钙接头分子1（Iba-1）的表达水平。结果与C组比较，R＋I组和M＋R＋I组T1-4时MWT降低，TwL缩短，脊髓GFAP和Iba-1的表达上调（P〈0．05），M组上述各指标差异无统计学意义（P〉0．05）。与R＋I组比较，M＋R＋I组T。时MWT升高，聊L延长，脊髓GFAP和Iba-1的表达下调（P〈0.05）。结论脊髓CCR5参与了瑞芬太尼诱发切口痛大鼠痛觉过敏，其机制可能与激活星形胶质细胞和小胶质细胞有关。Objective To evaluate the role of spinal C-C motif chemokine receptor 5 （CCR5） in remifentanil-induced hyperalgesia in rats with incisional pain. Methods Thirty-two male Sprague-Dawley rats, weighing 240-260 g, aged 2-3 months, in which intrathecal and caudal catheters were successfully implanted, were divided into 4 groups （ n = 8 each） using a random number table ： control group （ group C）, C CR5 antagonist maraviroe group （group M）, remifentanil plus incisional pain group （group R＋I） and maraviroe plus remifentanil plus incisional pain group （group M ＋R＋ I）. Phosphate buffer solution （PBS） 10 ~1 was intrathecally injected and normal saline was infused for 60 min at 1 μg·kg^-1·min^-1 via the caudal vein in group C. Maraviroc 100 pmol （in l0 Ixl of PBS） was intrathecally injected and normal saline was infused for 60 min at 1 μg·kg^-1·min^-1 via the caudal vein in group M. PBS 10 I^1 was intrath- ecally injected, then the model of incisional pain was established, and remifentanil 1μg·kg^-1·min^-1 was infused for 60 min via the caudal vein in group R＋I. Maraviroc 100 pmol （in 10 -1 of PBS） was in-trathecally injected, then the model of incisional pain was established, and remifentanil 1 μg·kg^-1·min^-1 was infused for 60 min via the caudal vein in group M＋R＋I. The mechanical paw with- drawal threshold （MWT） and thermal paw withdrawal latency （TWL） were measured at 24 h before infu- sion of remifentanil or normal saline （T0） and 2, 6, 24 and 48 h after stopping infusion （T1-4）. The rats were sacrificed after the last measurement of pain threshold, and L4.6 segments of the spinal cord were re- moved for determination of the expression of glial fibrillary acidic protein （GFAP） and ionized calcium- binding adapter molecule- 1 （ Iba- 1 ） by Western blot. nificantly decreased and TWL was shortened at T1_4, Results Compared with group C, the MWT was sig- and the expression of GFAP and Iba-1 in the spinal cord was up-regulated in R＋I

关 键 词：哌啶类 痛觉过敏 疼痛 手术后 受体 CCR5 脊髓 

分 类 号：R614[医药卫生—麻醉学]