洛贝林对慢性神经病理性疼痛的影响  

Effects of Lobeline on chronic neuropathic pain

作　　者：杨宁 伏杰 尹家乐 陈春江 徐天一 王雪 马涛 YANG Ning;FU Jie;YIN Jiole;CHEN Chunjiang;XU Tianyi;WANG Xue;MA Tao(School of Anesthesiology,Xuzhou Medical University,Jiangsu Province,Xuzhou 221004,China;Department of Anesthetic Pharmacology,Xuzhou Medical University,Jiangsu Province,Xuzhou 221004,China)

机构地区：[1]徐州医科大学麻醉学院,江苏徐州221004 [2]徐州医科大学麻醉药理教研室,江苏徐州221004

出　　处：《中国医药导报》2018年第27期17-20,共4页China Medical Herald

基　　金：江苏高校品牌专业建设工程项目(PPZY2015A066)

摘　　要：目的探讨洛贝林对慢性神经病理性疼痛的影响。方法采用随机数字表法将48只2月龄的小鼠分为4组,常规喂养。一组为空白对照组,其余三组均制备坐骨神经慢性缩窄性损伤模型(CCI):模型组(CCI+腹腔注射生理盐水),小剂量洛贝林组(CCI+腹腔注射3 mg/kg洛贝林溶液),大剂量洛贝林组(CCI+腹腔注射6 mg/kg洛贝林溶液)。应用Von Fery机械测痛法及旷场实验于造模后第1、8、22天给药后测定小鼠痛阈及活动程度。结果痛阈值的测定中,与空白对照组比较,模型组在造模后第1、8、22天均下降(P<0.05);与模型组比较,三次实验中大剂量洛贝林组均有升高(P<0.05)。旷场实验中,与空白对照组比较,模型组在第1、22天中总路程和平均速度显著降低(P<0.01);与模型组比较,大剂量洛贝林组于造模后第22天总路程和平均速度均有显著提高(P<0.01),活动次数明显提高(P<0.05)。结论 6 mg/kg洛贝林溶液能够使慢性神经病理性疼痛小鼠痛阈值升高,并且能够改善小鼠的抑郁样行为。Objective To investigate the effects of Lobeline on chronic neuropathic pain. Methods Forty-eight mice aged 2 months were divided into 4 groups by random number table method and fed routinely： one group was blank control group, and chronic constriction injury model of sciatic nerve were prepared in the other three groups; model group（CCI ＋ intraperitoneal injection of saline）, low dose Lobeline group（CCI ＋ intraperitoneal injection of 3 mg/kg Lobeline solution）, high dose Lobeline group（CCI ＋ intraperitoneal injection of 6 mg/kg Lobeline solution）. The pain threshold and activity level of mice were measured by Von Fery mechanical pain measurement and open field test on the 1 st, 8 th,and 22 nd days after injection. Results In the determination of pain threshold, the model group decreased on the 1 st,8 th, and 22 nd days after injection compared with the blank control group（P〈0.05）. While compared with the model group, the high dose Lobeline group was elevated in the three experiments（P〈0.05）. In the open field experiment,compared with the blank control group, the total distance and average speed of the model group significantly decreased on the 1 st and 22 nd days（P〈0.01）. Compared with the model group, the total distance and mean velocity of the high dose Lobeline group on the 22 th day increased significantly（P〈0.01）, and the number of activities was improved（P〈0.05）. Conclusion The 6 mg/kg Lobeline solution can increase the threshold of mice with chronic neuropathic pain and improve the mice′s depression-like behavior.

关 键 词：洛贝林 神经病理性疼痛 抑郁 旷场实验 痛阈 

分 类 号：R741[医药卫生—神经病学与精神病学]