基于肠道菌群探讨保和丸对高脂饮食SD大鼠血脂的作用  

Effects of Baohe Pills on Blood Lipid in SD rats with a High-fat Diet on the Basis of Intestinal Microbiota

作　　者：李玉波[1] 马雪玲[2] 李志更[1] 贾海骅[1] 梁媛[1] 郝改梅 Li Yubo;Ma Xueling;Li Zhigeng;Jia Haihua;Liang Yuan;Hao Gaimei(Institute of Basic Theory for Chinese Medicine,China Academy of Chinese Medicine Science,Beijing 100700,China;Beijing University of Chinese Medicine,Beijing 102446,China)

机构地区：[1]中国中医科学院中医基础理论研究所,北京100700 [2]北京中医药大学,北京102446

出　　处：《世界中医药》2018年第9期2107-2110,2116,共5页World Chinese Medicine

基　　金：国家自然科学基金青年基金项目(81603417)--"抑郁症肝郁致脾虚的中枢海马-下丘脑调控机制研究";中央级公益性科研院所基本科研业务费中国中医科学院基础所自主选题项目(YZ-1539)--"加味越鞠丸对动脉粥样硬化的作用机制研究"

摘　　要：目的:观察保和丸对高脂饮食SD大鼠血脂和肠道菌群的影响,为临床应用和实验研究提供参考依据。方法:将60只大鼠随机分为4组:正常组、模型组、高脂饮食保和丸组(简称"高保组")、正常饮食保和丸组(简称"正保组"),Elisa法检测大鼠血清血脂含量,基于16Sr DNA基因测序技术分析大鼠肠道菌群。结果:高保组大鼠血清总胆固醇(TC)、三酰甘油(TG)和低密度脂蛋白(LDL-C)含量与模型组比较明显减少但仍高于正常组和正保组,正保组与正常组比较,差异无统计学意义(P>0.05)。肠道菌群分析结果显示正保组与正常组大鼠比较,其粪便样本微生物菌群数量无明显差异。高保组与模型组比较能显著增加纲双歧杆菌目、梭菌目、脱硫弧菌目、产氢细菌目菌群数量,但仍低于正常组和正保组;同时能显著减少红蝽菌目、拟杆菌目、芽孢肝菌目、乳杆菌目、丹毒丝菌目、伯克氏菌目、气单胞菌目、疣微菌目菌群数量,但仍高于正常组和正保组。结论:保和丸可能通过改变肠道菌群多样性来降低TC、TG和LDL-C。Objective： To observe the effects of Baohe Pills on blood lipid and intestinal microbiota in SD rats with a high-fat diet to provide reference for clinical application and experimental research. Methods： A total of 60 rats were randomly divided into 4 groups： normal group,model group,high-fat diet with Baohe pills group（ HB group） and the normal diet with Baohe pills group（ NB group）. The serum lipid level was detected by Elisa method,and the intestinal microbiota of rats was analyzed based on 16 S r DNA gene sequencing technique. Results： the contents of serum cholesterol（ TC）,triglyceride（ TG） and low-density lipoprotein（ LDL-C） in the rats of HB group were decreased more significantly than those of the model group,but less than those of the normal group and the NB group. There was no statistically significant difference between the normal group and the NB group. The results of intestinal microbiota analysis showed no significant difference in number of fecal microbial flora between the NB group and the normal group. Compared with the model group,the numbers of Bifidobacteriales,Clostridiales,Desulfovibrionales,and RF9 bacteria of HB group decreased more than that of the normal group and the NB group. In the meantime,compared to the model group,the HB group experienced a significantly reduction in the number of Coriobacteriales,Bacteroidales,Bacillales,Lactobacillales,Erysipelotrichales,Burkholderiales,Aeromonadales,Verrucomicrobiales,etc.,but the number was still higher than that of the normal group and the NB group. Conclusion： Baohe pills may reduce TC,TG and LDL-C by altering the diversity of intestinal microbiota.

关 键 词：肠道菌群 保和丸 高脂饮食 血脂 

分 类 号：R285.5[医药卫生—中药学]