胰腺癌组织中人磷脂酰乙醇胺结合蛋白4的表达及临床意义  

Expression of human phosphatidylethanolamine-binding protein 4 in pancreatic cancer tissue and its clinical significance

作　　者：张生彬[1] 陈冰[1] 董子龙[1] 刘瑞 云哲琳[1] ZHANG Shengbin, CHEN Bing, DONG Zilong, LIU Rui, YUN Zhelin(Department of Hepatobiliary Surgery, Baogang Hospital of Inner Mongolia, Baotou, Inner Mongolia 014010, China)

机构地区：[1]内蒙古包钢医院肝胆外科,内蒙古包头014010

出　　处：《中国普通外科杂志》2018年第9期1120-1125,共6页China Journal of General Surgery

基　　金：内蒙古自治区卫计委医疗卫生科研计划资助项目(201301080)

摘　　要：目的:探讨人磷脂酰乙醇胺结合蛋白4(hPEBP-4)在胰腺癌中的表达及临床意义。方法:采用qRT-PCR、Western blot和免疫组化方法检测30例胰腺癌组织及其癌旁组织中hPEBP-4的mRNA与蛋白表达,分析hPEBP-4表达水平与胰腺癌患者临床病理参数和预后的关系。结果:qRT-PCR与Western blot结果显示,相对于癌旁组织,30例胰腺癌组织中hPEBP-4的mRNA与蛋白表达上调者24例(80.0%);免疫组化结果显示,hPEBP-4在胰腺癌组织中的高表达率明显高于癌旁组织(80.0%vs. 16.7%,χ~2=24.093,P<0.001)。hPEBP-4的表达水平与患者的肿瘤分化程度、CA19-9水平以及TNM分期有关(均P<0.05)。相关性结果显示,胰腺癌分化程度、TNM分期均与hPEBP-4呈负相关(r=-0.507,P=0.004;r=-0.400,P=0.028),而血清CA19-9水平与hPEBP-4呈正相关(r=0.428,P=0.018)。生存分析结果显示,hPEBP-4低表达患者的总生存率明显高于高表达患者(χ~2=8.658,P=0.003)。结论:hPEBP-4在胰腺癌组织中表达升高,且与胰腺癌患者的部分恶性病理特征及不良预后密切相关。Objective： To investigate the expression of human phosphatidylethanolamine-binding protein 4 （hPEBP-4） in pancreatic cancer tissue and its clinical significance. Methods： The mRNA and protein expressions ofhPEBP-4 in specimens of pancreatic cancer and paired adjacent tissues from 30 patients were determined by qRT-PCR, Western blot and immunohistochemical staining, respectively. The relations of the hPEBP-4 expression with clinicopathologic variables and prognosis of the pancreatic cancer patients were analyzed. Results： The results ofqPCR and Western blot showed that the mRNA and protein expressions of hPEBP-4 were obviously up-regulated in 24 specimens of the 30 pancreatic cancer tissues relative to their adjacent tissues. The results of immunohistochemical staining showed that the high expression rate of hPEBP-4 in pancreatic cancer tissues was significantly higher than that in adjacent tissues （80.0% vs. 16.7%, x2=24.093, P〈0.001）. The expression level of hPEBP-4 was significantly related to the degree of tumor differentiation, serum CA19-9 level and TNM stage （all P〈0.05）. The results of correlation analysis showed that the differentiation degree of pancreatic cancer and TNM staging were negatively correlated with hPEBP-4 （r=-0.507, P=0.004; r=-0.400, P=0.028）, while serum CA19-9 level was positively correlated with hPEBP-4 （r=0.428, P=0.018）. The results of survival analysis showed that the overall survival rate in patients with low hPEBP-4 expression was significantly higher than that in patients with high hPEBP-4 expression （Xz=8.658, P=0.003）. Conclusion： The expression of hPEBP-4 is increased in pancreatic cancer tissue, which is associated with some malignant pathological features and poor prognosis in pancreatic cancer.

关 键 词：胰腺肿瘤 磷脂酰乙醇胺结合蛋白质 肿瘤侵润 预后 

分 类 号：R735.9[医药卫生—肿瘤]