磁场条件下Fe^(2+)对产聚唾液酸大肠杆菌的诱变选育  

Title Screening of high-yield polysialic acid producing Escherichia coli mutants induced by magnetic field combined with Fe^(2+)

作　　者：吴金勇 陈祥松[2,3] 袁丽霞[2] 朱薇薇 孙立洁[2] 李俊[3] 高兰兰[2] 姚建铭 Wu Jinyong;Chen Xiangsong;Yuan Lixia;Zhu Weiwei;Sun Lijie;Li Jun;Gao Lanlan;Yao Jianming(University of Chinese Academy of Sciences,Beijing 100049,China;Hefei institutes of Physical Science,Chinese Academy of Sciences,Hefei 230031,China;Huainan New Energy Research Center,Huainan,232001,China)

机构地区：[1]中国科学院大学,北京100049 [2]中国科学院合肥物质科学研究院,合肥230031 [3]淮南新能源研究中心,安徽淮南232001

出　　处：《河南师范大学学报（自然科学版）》2018年第6期80-85,共6页Journal of Henan Normal University(Natural Science Edition)

基　　金：国家自然科学基金(215062351008217);淮南市重点研究和开发专项(2017A044);中科院等离子体物理研究所科学基金(DSJJ-16-YY02)

摘　　要：以产聚唾液酸(polysialic acid,PSA)的大肠杆菌(Escherichia coli)为出发菌株,在磁场条件下利用Fe^(2+)对该菌种进行多轮诱变选育,获得具有高产性能的诱变菌株,在5L发酵罐进行发酵验证.结果表明:在0.5T磁场条件下,Fe^(2+)的最佳诱变质量浓度为90mg·L^(-1);经过5轮诱变和选育,PSA的摇瓶发酵水平从1.09g·L^(-1)提高到2.24g·L^(-1);5L发酵罐发酵水平从4.79g·L^(-1)提高到9.22g·L^(-1),产量提高了92%.Compound mutagenesis using magnetic field and Fe 2＋ was carried out several rounds to gain a high-yielding Escherichia coli strain of polysialic acid （PSA） fermentation and the high-yielding strain was verified in 5 L fermentor. The results showed that the optimal Fe 2＋ ion concentration was 90 mg·L -1 in the compound mutagenesis using magnetic field of 0.5 T . The concentration of PSA in shaking flask fermentation in creased from 1.09 g·L -1 to 2.24 g·L -1 after five rounds of mutagesis, while the concentration of PSA in 5L fermentor fermentation raised from 4.79 g·L -1 to 9.22 g·L -1 , increased by 92%. Therefore, the compound mutagenesis of magnetic field and Fe 2＋ showed obvious effect on Escherichia coli of PSA fermentation.

关 键 词：聚唾液酸 磁场 亚铁离子 诱变 

分 类 号：Q939.9[生物学—微生物学]