重金属离子对不动杆菌BCL-1降解吡虫啉的影响  

Effect of Imidacloprid Degrading Strain BCL-1 by Heavy Metal Ions

作　　者：胡桂萍[1,2,3] 曹红妹 石旭平 杨帆[1,3] 施龙清 杜贤明[1,3] 王军文 邓真华 王礼献[1,3] 江新凤 HU Gui-Ping;CAO Hong-Mei;SHI Xu-Ping;YANG Fan;SHI Long-Oing;DU Xian-Ming;WANG Jun-Wen;DENG Zhen-Hua;WANG Li-Xian;JIANG Xin-Feng(Jiangxi Serieulture and Tea Research Institute,Nanehang 330202;State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops,Institute of Applied Ecology,Fujian Agriculture and Forestry University,Fuzhou 350002;Research Center of Jiangxi Silk and Mulberry Engineering ＆ Technology,Nanchang 330202,China)

机构地区：[1]江西省蚕桑茶叶研究所,江西南昌330202 [2]闽台作物有害生物生态防控国家重点实验室,福建农林大学应用生态研究所,福建福州350002 [3]江西省蚕桑工程技术研究中心,江西南昌330202

出　　处：《生物技术通讯》2018年第5期619-624,共6页Letters in Biotechnology

基　　金：政府间国际科技创新合作重点专项(2016YFE0102100);闽台作物有害生物生态防控国家重点实验室开放课题(skb2017011);江西省重点研发项目(20161BBH80071)

摘　　要：目的:进一步明确吡虫啉降解菌不动杆菌BCL-1的降解动力学特性,以及重金属离子对其降解特性的影响。方法:实验分析菌株BCL-1对100 mg/L吡虫啉的降解动力学特性和半衰期,以及不同Cu^(2+)和Al^(3+)浓度的影响。结果:降解菌BCL-1对100 mg/L吡虫啉的降解率在第7 d达到最高,为87.9%,而菌株生长量第5 d达到最高,此条件下的降解动力学模型为y=55.175e^(-0.271x)(R^2=0.8179),半衰期为2.56 d。在10、50、100、200、500 mg/kg Cu^(2+)影响下,BCL-1第5 d对吡虫啉的降解率分别为59.84%,62.49%,62.96%,66.26%,64.84%,其对应的半衰期分别为2.16、2.06、2.14、2.08、2.12 d,均与同期空白对照的变化趋势一致且无差异;在10、50、100 mg/kg Al^(3+)影响下,BCL-1第5 d对吡虫啉的降解率分别为79.51%,75.56%和73.97%,半衰期分别为1.52,1.56,1.75 d,比同期对照高;在500、1000 mg/kg Al^(3+)影响下,BCL-1第5 d对吡虫啉的降解率分别为34.14%和36.18%,半衰期分别为3.67和3.52 d,同期均低于对照。结论:不同浓度的Cu^(2+)对菌株BCL-1降解吡虫啉无影响;低于100 mg/L的Al^(3+)可促进吡虫啉的降解,高于200mg/kg的Al^(3+)会抑制吡虫啉的降解,进一步表明菌株BCL-1吡虫啉降解与环境酸碱度有关。Objective： To clarify the degradation kinetics of imidacloprid degradation bacteria BCL-1 and the effect of heavy metal ions on its degradation characteristics. Methods： The degradation kinetics and half-life of imidacloprid（100 mg/L） by strain BCL-1 and effects of different concentrations of Cu^2＋ and Al^3＋ were analyzed in this study. Results： The highest degradation rate of 87.9% reached on the 7th day after inoculating strain BCL-1 on the mediunl with the imidaclorpid of 100 mg/L, and the highest biomass were gained in the 5th day, and the degradation kinetics model of y=55.175e^-0.271x with a con＇elation coefficient of R^2=0.8179 and the half-life（t1/2） of 2.56 days were received under such conditions. And then, when adding different concentration of Cu〉（10, 50, 100, 200 and 500 mg/kg） respectively, the 5th day degradation rate of strain BCL-1 reached 59.84%, 62.49%, 62.96%, 66.26%, 64.84%, with its corresponding t1/2 respectively 2.16, 2.06, 2.14, 2.06, 2.14 days, which was consistent with that of the blank control group. But Al^3＋ concentration of 10, 50 and 100 mg/kg, the degradation rate of BCL-1 strain on the 5th day was 79.51%, 75.56% and 73.97% respectively, and the t1/2 was 1.52, 1.56 and 1.75 days respectively, after adding Al^3＋ concentration of 10, 50 and 100 mg/kg, respectively, which were higher than the control at the same time. But the influence of AP＋ with concentration of 500 and 1000 mg/kg showed that the degradation rate of imidacloprid on the 5th day was 34.14% and 36.18% respectively, and the t1/2 was 3.67 and 3.52 days respectively, both of which were lower than the control at the same time. Conclusion： No effect of imidacloprid degradation of strains BCL-1 by the different concentration of Cu^2＋, but less than 100 mg/L of Al^3＋ ＋could promote the degradation of imidacloprid, and higher than 200 mg/kg of Al^3＋ could inhibit the degradation of imidacloprid, that also demonstating imidacloprid degradation by strain BCL-1 were related with the pH i

关 键 词：不动杆菌 吡虫啉降解菌 重金属离子 降解动力学 

分 类 号：Q939.9[生物学—微生物学]