CDX2基因通过NF-κB信号通路抑制结肠癌细胞增殖  

CDX2 gene inhibits of colon cancer cell proliferation by NF-κB signaling pathway

作　　者：陈鳞 王恩湘[1,2] 刘群友 钟鹰[1,2] 周国良 赵喜雁[1,2] CHEN Lin;WANG Enxiang;LIU Qunyou;ZHONG Ying;ZHOU Guoliang;ZHAO Xiyan(The First People′s Hospital of Xiangtan City,The Xiangtan Hospital of South China University,Xiangtan 411101,China)

机构地区：[1]湘潭市第一人民医院 [2]南华大学附属湘潭医院,湘潭411101

出　　处：《实用肿瘤学杂志》2018年第5期398-403,共6页Practical Oncology Journal

摘　　要：目的观察过表达CDX2基因对人结肠癌细胞株增殖影响,并初步探讨其作用机制。方法通过基因转染技术将CDX2基因转染入结肠癌HT-29细胞株,经G418筛选后获得稳定转染细胞株,RT-PCR和Western blot检测CDX2基因表达。采用四甲基偶氮唑盐(MTT)法、流式细胞仪分析转染细胞的生物学行为。Western blot检测CDX2过表达对HT-29细胞内Cyclin D1和NF-κB磷酸化水平的影响。结果经脂质体转染和筛选,建立了稳定过表达CDX2人结肠癌HT-29细胞株。转染CDX2组与未转染组和空白质粒组相比,细胞的生长速度减慢(P <0. 05),细胞周期中G1/G0期比例增加(P <0. 05),而S期比例则减少(P <0. 05);与未转染组和空白质粒组比较,转染CDX2基因组HT-29细胞的Cyclin D1和p-NF-κB活性明显降低。结论 CDX2基因可能通过抑制NF-κB通路抑制Cyclin D1活性,从而抑制人结肠癌HT-29细胞增殖。Objective The aim of this study was to observe the effects of overexpressing CDX2 gene on proliferation of human colon cancer HT-29 cell line and to explore its mechanism.Methods CDX2 was transfected into HT-29 cells by gene transfection technology,and stable transfected cell clones with stably expressing CDX2 gene were obtained by G418 selection.The expression of CDX2 was detected by RT-PCR and Western blotting.The biological behavior of transfected cells were analyzed by methythiazoletertraolium（MTT）assay and flow cytometry.Western blot assay was used to detect the expression of cyclin D1 and NF-κB phosphorylation level in HT-29 cells with overexpressing CDX2 gene.Results A system for stably overexpressing CDX2 human colon cancer HT-29 cell line was established.Compared with the nontransfected and empty vector transfected HT-29 cells,the growth rate of the cells in the transfected CDX2 group was significantly slower（P〈0.05）.The proportion of G1/G0 phase of cell cycle was significantly increased（P〈0.05）,and the proportion of S phase was significantly decreased（P〈0.05）.Compared with the non-transfected and empty vector transfected cells,the expression of cyclin D1 and p-NF-κB activity were significant low in overexpressing CDX2 cells.Conclusion CDX2 gene may inhibit the proliferation of HT-29 cells through blocking the activity of NF-κB pathway and down-regulation of cyclin D1 protein expression.

关 键 词：结肠癌 细胞增殖 CDX2 NF-ΚB 

分 类 号：R735.3[医药卫生—肿瘤]