HLA-G在乳腺癌中的表达及意义  

The expression of HLA-G in breast cancer and its significance

作　　者：何煦 叶尚勉[1,2] 董丹丹[1,3] 李科[1,3] HE Xu;YE shangmian;DONG dandan;LI Ke(Core Laboratory,Sichuan Academy of Medical Sciences,Sichuan Provincial People′s Hospital,Chengdu 610072,China;Sichuan Academy of Medical Sciences and the department of pathology,Sichuan Provincial People′s Hospital)

机构地区：[1]四川省医学科学院 [2]四川省人民医院中心实验室,成都610072 [3]四川省人民医院病理科

出　　处：《实用肿瘤学杂志》2018年第5期410-414,共5页Practical Oncology Journal

基　　金：四川省卫计委科研资助项目(编号:120106)

摘　　要：目的探讨乳腺癌患者组织中人类白细胞抗原G(Human leukocyte antigen,HLA-G)的表达及其在乳腺癌诊断和预后中的作用。方法分离纯化HLA-G蛋白,筛选出分泌抗HLA-G单克隆抗体的杂交瘤细胞株,得到抗HLA-G单克隆抗体。用免疫组织化学方法检测235例乳腺癌患者组织中HLA-G的表达,分析HLA-G的表达与各项临床病理指标、宿主免疫反应及生存率之间的关系。结果 HLA-G在乳腺癌患者的表达率为66%(155/235),HLA-G表达与肿瘤大小、临床TNM分期以及淋巴结转移有关。HLA-G阴性病人的生存率明显高于HLA-G阳性病人。结论乳腺癌组织中HLA-G有较高表达,HLA-G的表达与宿主免疫反应及乳腺癌的预后有关,HLA-G的表达可能是影响乳腺癌病人预后的独立因素。Objective The Objective of this study was to investigate the expression of human leukocye antigen-G（HLA-G）in breast cancer patients and its role in the diagnosis and prognosis of breast cancer.Methods HLA-G protein was isolated and purified,and hybridoma cell lines secreting anti-HLA-G monoclonal antibody were screened to obtain anti-HLA-G monoclonal antibody.The expression of HLA-G in 235 primary breast cancer tissues was detected by immunohistochemistry.The relationship amongst HLA-G expression with various clinicopathological parameters,host immune response ＆ survival rate was analyzed.Results The expression rate of HLA-G in breast cancer patients was 66%（155/235）.The expression of HLA-G was related to tumor size,clinical TNM stage and lymph node metastasis.The survival rate of HLA-G negative patients was significantly higher than that of HLA-G positive patients.Conclusion HLA-G is highly expressed in breast cancer tissues.The expression of HLA-G is related to host immune response and prognosis of breast cancer.HLA-G is an independent factor affecting the prognosis of breast cancer patients.

关 键 词：人类白细胞抗原G(HLA-G) 乳腺癌 免疫组织化学 

分 类 号：R737.9[医药卫生—肿瘤]