碱性木聚糖酶产生菌株的分离鉴定与产酶分析  

Isolation and Identification of an Alkaline Xylanase-Producing Bacterium and Enzymatic Analysis

作　　者：甘雅霆 周慧 李艳 董重实 赵硕 闫达中 GAN Yating;ZHOU Hui;LI Yan;DONG Zhongshi;ZHAO Shuo;YAN Dazhong(School of Biology and Pharmaceutical Engineering,Wuhan Polytechnic University,Wuhan 430023,China;College of Resources and Environment,University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]武汉轻工大学生物与制药工程学院,湖北武汉430023 [2]中国科学院大学资源与环境学院,北京100049

出　　处：《食品科学》2018年第20期155-160,共6页Food Science

基　　金：湖北省自然科学基金项目(2008CDB067)

摘　　要：目的:分离1株高产碱性木聚糖酶菌株。方法:以木聚糖为唯一碳源,从造纸厂活性污泥中筛选1株高产碱性木聚糖酶细菌,采用3,5-二硝基水杨酸法定糖法测定菌株产木聚糖酶的活力,并通过生理、生化特征以及16S rRNA基因序列分析比对对菌株进行鉴定。结果:菌株YD01所产木聚糖酶的最适pH值为8.0,最适温度为50℃;在pH 10和80℃时,仍有87%和85%的剩余酶活力,具有较好的耐碱性及较高的热稳定性。最终发酵产酶水平为36.85 U/mL。结论:鉴定该菌株为蛾微杆菌(Microbacterium imperiale),所产生的木聚糖酶具有较高的碱耐受性和热稳定性。Objective： This study aimed to isolate a strain with high alkaline xylanase activity. Methods： The target strain was isolated with xylan as the only carbon source from the paper mill sludge. The xylanase activity was determined by the 3,5-dinitrosalicylic acid （DNS） method. The strain was identifed based on physiological, biochemical and 16S rRNA gene sequence analysis. Results： The optimal pH of the xylanase enzyme was 8.0, and the optimum temperature was 50 ℃. The enzyme retained 87% and 85% of its initial activity at pH 10 and 80 ℃, respectively. It proved to be highly stable to both alkali and heat. The xylanase activity of the fermentation broth of the strain was 36.85 U/mL. Conclusion： This strain was identifed as Microbacterium imperiale, which could produce a thermo-alkali-stable xylanase.

关 键 词：碱性木聚糖酶 蛾微杆菌 鉴定 

分 类 号：Q939.9[生物学—微生物学]