新疆野生樱桃李(Prunus cerasifera)叶黄酮类成分研究  

Study on the Flavonoids in the Leaves of Prunus cerasifera Grown in Xinjiang

作　　者：刘伟[1] 李紫薇[1] 王航 杨凤玲 李明玺 刘晓[1] 万春鹏 LIU Wei;LI Zi-wei;WANG Hang;YANG Feng-ling;LI Ming-xi;LIU Xiao;WAN Chun-peng(Yili Normal University Key Laboratory at Universities of Education Department of Xinjiang Uygur Autonomous Region,Yining 835000,China;College of Agronomy,Jiangxi Agricultural University,Jiangxi Key Laboratory for Post-harvest Technology and Nondestructive Testing of Fruits ＆ Vegetables / Collaborative Innovation Center of Post-harvest Key Technology and Quality Safety of Fruits and Vegetables in Jiangxi Province,Nanchang 330045,China)

机构地区：[1]伊犁师范学院,自治区教育厅普通高校重点实验室,新疆伊宁835000 [2]江西农业大学农学院,江西省果蔬保鲜与无损检测重点实验室,江西省果蔬采后处理关键技术与质量安全协同创新中心,江西南昌330045

出　　处：《现代食品科技》2018年第9期263-267,290,共6页Modern Food Science and Technology

基　　金：新疆维吾尔自治区自然科学基金项目(2017D01C421)

摘　　要：此项目研究了新疆野生樱桃李叶乙酸乙酯活性部位中的黄酮类化学成分。采用甲醇浸泡提取野生樱桃李叶,依次采用环己烷、乙酸乙酯和正丁醇萃取其醇提物。乙酸乙酯活性部分采用MCI (Middle Chromatogram Isolated Gel)大孔树脂柱粗分离,后续进一步采用硅胶柱、Sephadex LH-20凝胶柱,ODS (Octadecyl silane)反向硅胶柱和制备HPLC等色谱技术对其进行分离纯化,并根据化合物理化性质和核磁共振波谱(NMR)数据并与相关参考文献比对鉴定化合物结构。从野生樱桃李叶中共分离并鉴定11个黄酮类化合物,分别为:槲皮素-3-O-α-L-呋喃阿拉伯糖苷(1),槲皮素-3-O-β-D-木糖苷(2),槲皮素-3-O-β-D-半乳糖苷(3),槲皮素-3-O-α-L-鼠李糖苷(4),山柰酚-3-O-α-L-呋喃阿拉伯糖苷(5),山柰酚-3-O-β-D-木糖苷(6),山柰酚-3-O-β-D-葡萄糖苷(7),山柰酚-3-O-α-L-鼠李糖苷(8),反式-二氢山柰酚-3-O-α-L-鼠李糖苷(9),顺式-二氢山柰酚-3-O-α-L-鼠李糖苷(10),顺式-二氢山柰酚-3-O-β-D-葡萄糖苷(11)。所有黄酮类成分均为首次从新疆野生樱桃李叶中分离鉴定,槲皮素和山柰酚糖苷是其主要的黄酮类成分。This study analyzed the chemical constituents of flavonoids in the active fraction of ethyl acetate in the leaves of Prunus cerasifera. The leaves were firstly soaked in methanol and the alcoholic extract was extracted sequentially using cyclohexane, ethyl acetate and n-butanol. The active fraction of ethyl acetate was roughly separated by MCI（Middle Chromatogram Isolated Gel） macroporous resin column, followed by further purification by chromatography using silica gel column, Sephadex LH-20 gel column, ODS（Octadecyl silane） reverse silica gel column and preparative HPLC. The isolated flavonol constituents were identified by 1 H-NMR and 13 C-NMR analyses. Identification of compounds based on their physicochemical properties and nuclear magnetic resonance spectroscopy（NMR） data and through comparison with related references. Eleven flavonol flavonoids were isolated and identified： quercetin-3-O-α-L-arabinoside（1）, quercetin-3-O-β-D-xyloside（2）, quercetin-3-O-β-D-galactoside（3）, quercetin-3-O-α-L-rhamnoside（4）, kaempferol-3-O-α-L-arabinoside（5）, kaempferol-3-O-β-D-xyloside（6）, kaempferol-3-O-β-D-glucoside（7）, kaempferol-3-O-α-L-rhamnoside（8）, trans-dihydrokaempferol-3-O-α-L-rhamnoside（9）, cis-dihydrokaempferol-3-O-α-L-rhamnoside（10）, cis-dihydrokaempferol-3-O-β-D-glucoside（11）. All flavonoids were isolated and identified from the leaves of P. cerasifera for the first time, with quercetin and kaempferol glycosides being the major flavonoids.

关 键 词：野生樱桃李 黄酮 活性成分 

分 类 号：R284.1[医药卫生—中药学]