姜黄素抑制乳腺癌细胞增殖与侵袭机制探讨  

Mechanism of curcumin inhibiting proliferation and invasion of breast cancer cells

作　　者：亓小改 韩翔[1] 郝娟芝[1] 杨萍萍[1] 邢晓波[1] 杨现松[1] QI Xiao-gai;HAN Xiang;HAO Juan-zhi;YANG Ping-ping;XING Xiao-bo;YANG Xian-song(Department of Radiotherapy,Second Affiliated Hospital of Qingdao University,Qingdao 266042,P.R.China)

机构地区：[1]青岛大学医学院第二附属医院放疗科,山东青岛266042

出　　处：《中华肿瘤防治杂志》2018年第17期1211-1216,共6页Chinese Journal of Cancer Prevention and Treatment

摘　　要：目的姜黄素是印度香料姜黄中主要的姜黄类化合物。姜黄素在肿瘤增殖和侵袭方面的作用越来越受到重视。本研究探讨姜黄素对乳腺癌MDA-MB-231细胞增殖与侵袭的影响及其机制。方法用不同浓度姜黄素处理MDA-MB-231细胞,采用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐[3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide,MTT]法检测细胞增殖,Transwell小室法观察姜黄素对MDA-MB-231细胞侵袭能力的影响,FCM结合PI及AnnexinⅤ-FITC双标记染色测定细胞凋亡,Hoechst染色观察细胞形态变化。逆转录-聚合酶链反应(reverse transcription-polymerase chain reaction,RT-PCR)和蛋白质印迹法分别检测PTN、MMP-9mRNA和蛋白表达变化。结果姜黄素30μmol/L作用MDA-MB-231细胞24、48和72h,细胞增殖抑制率分别为(24.20±0.91)%、(36.78±0.69)%和(37.41±0.41)%,姜黄素60μmol/L作用24、48和72h,细胞增殖抑制率分别为(37.41±0.41)%、(46.49±0.80)%和(53.84±1.50)%。各浓度组不同作用时间差异有统计学意义,F=17.327,P<0.001;不同浓度组之间差异有统计学意义,F=861.916,P<0.001;两者间存在协同交互效应。DMSO对照组与姜黄素30、60μmol/L作用MDA-MB-231细胞24h后,穿膜细胞数分别为(212.40±13.61)、(146.80±13.25)和(98.60±8.23)个/视野,穿膜细胞数呈剂量依赖性明显减少,细胞侵袭能力受抑,差异有统计学意义,F=959.002,P<0.001;DMSO对照组与经过5、10、20、30和60μmol/L不同浓度的姜黄素处理24h后,各组坏死和凋亡晚期细胞百分率分别为(6.04±0.98)%、(8.71±1.15)%、(11.04±1.04)%、(20.97±1.20)%、(46.87±1.24)%和(56.31±1.87)%,差异有统计学意义,F=836.251,P<0.001;姜黄素0(DMSO对照组)、30和60μmol/L作用乳腺癌MDA-MB-231细胞24h后,PTN mRNA相对比值分别为0.46±0.08、0.31±0.03和0.19±0.03,差异有统计学意义,F=20.848,P=0.002;MMP-9mRNA相对比值分别为0.88±0.05、0.54±0.07和0.31±0.02,差异有统计学意义,F=91.370,P<0.001。姜黄OBJECTIVE Curcumin is the main turmeric compound in Indian spice turmeric and more and more at- tention has been paid to its role in tumor proliferation and invasion. This research investigated the effect of curcumin on proliferation and invasion of breast cancer MDA-MB-231 cells and its mechanism. METHODS MDA-MB-231 cells were treated by different concentrations of curcumin. Cell proliferation was detected by 3-（4,5-dimethylthiazo-2）-2,5-dipheny- hertrazoliumromide （MTT assay）. Transwell microscopy was used in observing the effect of curcumin on invasiveness of MDA-MB-231 cells. Cell apoptosis and Hoechst staining were detected by FCM in combination with PI and Annexin V-FITC double labeling staining to observe cell morphological changes. RT-PCR and Western blot technique was respectively used to detected the PTN,MMP-9,mRNA and protein expression changes. RESULTS MDA-MB-231 cell proliferation inhibi- tion rates were （24.20±0.91） %, （36.78 ±0.69） % and （37.41 ±0.41） %by 30 μmol/L of curcumin for 24,48 and 72 h. MDA-MB-231 cell proliferation inhibition rates were （37.41 ± 0. 41） %, （46. 49 ± 0. 80）% and （53.84 ± 1.50） % by 60 μmol/L of curcumin for 24,48 and 72 h. There was statistically significant difference in different action time of eachconcentration group （F=17. 327,P〈0. 001）. The difference between different concentration groups was statistically sig nificant （F=861. 916,P〈0. 001）. There was a synergistic interaction effect between the lwo. MDA-MB-231 cell was treated by DMSO matched group and 30,60 μmol/L of different concentrations of curcumin for 24 h,the number of trans menbrane cells respectively was （212.40± 13.61）,（146.80±13.25） and （98.60±8.23）/horizon. The number of trans membrane cells was significantly decreased in a dose-dependent manner and the cell invasion ability was inhibited （F=959. 002,P〈0.00]）. Cells were treated by DMSO matched group and 5,10,20,30,60μmol/L of different concentrations of curcumin for 24 b. Necros

关 键 词：姜黄素 多效因子 乳腺癌 侵袭 

分 类 号：R737.9[医药卫生—肿瘤]