建立裸鼠FaDu喉咽癌原位种植瘤模型及其CT和病理研究  

Establishing an in situ implantation tumor model of FaDu laryngopharyngeal carcinoma in nude mice and its CT and pathological study

作　　者：娄可心[1] 罗守华[2] 张园[3] 顾宁[2] 刘方舟[3] LOU Kexin;LUO Shouhua;ZHANG Yuan;GU Ning;LIU Fang-zhou(Department of Pathology,Jiangsu Cancer Hospital,Nanjing 210009,China;School of Biological Science and Medical Engineering,Southeast University,Nanjing 210008,China;Department of Head＆ Neck Surgery,Jiangsu Cancer Hospital,Nanjing 21009,China)

机构地区：[1]江苏省肿瘤医院病理科,江苏南京210009 [2]东南大学生物科学与医学工程学院,江苏南京210008 [3]江苏省肿瘤医院头颈科,江苏南京210009

出　　处：《中国肿瘤外科杂志》2018年第5期311-314,共4页Chinese Journal of Surgical Oncology

基　　金：江苏省"六大人才高峰项目"(2017 WSN-053)

摘　　要：目的建立Fa Du喉咽癌原位种植瘤模型并对其CT及病理诊断进行分析。方法 Fa Du肿瘤细胞株复苏培养传代建立荷瘤裸鼠,在裸鼠喉咽部注入,建立Fa Du喉咽癌原位种植瘤模型。结果裸鼠喉咽部接种Fa Du肿瘤细胞悬液,2周后可成功建立裸鼠喉咽肿瘤模型,成瘤率100%(30/30),动物CT检查能明确发现肿瘤占位,HE染色证实肿瘤组织为中至高分化鳞状细胞癌。结论 Fa Du细胞悬液喉咽癌接种建立的裸鼠头颈部肿瘤模型具有建模周期短,稳定性好、易于重复、移植瘤成功率高、操作简单等特点。Objective To establish an in situ implantation tumor model of FaDu laryngopharyngeal carcinoma,then to observe the growth characteristics of tumor in the animal models,according to the performance of imagination and the results of the pathology.Methods We established tumor-bearing nude mouse after FaDu tumor cell lines recovery in culture and passages and made the cell suspension.Cell suspension was injected into the nude mouse＇s laryngopharynx,then,passage nude mouse were formed.Results At 2 weeks after injection of FaDu tumor cell suspension,the FaDu tumor models of the head and neck were made successfully in nude mouse,and the tumor formation rate was 100%（30/30） at the laryngopharynx.The micro CT scan detected the tumor at laryngopharynx.Hematoxylin-eosin staining showed that FaDu tumor tissues were confirmed as moderately to highly differentiated squamous cell carcinoma.Conclusions These findings indicate that tumor-bearing nude mouse models of the laryngopharynx established by FaDu cell suspension are characterized by short modeling cycle,good stability,easy to repeat,high tumor formation rate and simple operation.

关 键 词：种植 裸鼠模型 耳鼻咽喉肿瘤 CT 病理学 

分 类 号：R739.6[医药卫生—肿瘤] R-332[医药卫生—临床医学]