Twist和Vimentin蛋白在口腔鳞状细胞癌中的表达及其意义  

Significance of the levels of Twist and Vimentin proteins in oral squamous cell carcinoma

作　　者：高文峰 王治平 许竞 宋治锋 曾曙光 GAO Wenfeng;WANG Zhiping;XU Jing;SONG Zhifeng;ZENG Shuguang(Department of oral and maxillofaeial surgery,Stoma-tologieal Hospital,Southern Medical University,Guangzhou 510280,China;Shanghai Stomatological Hospital,Shang-hai 200001,China)

机构地区：[1]南方医科大学口腔医院口腔颌面外科,广东广州510280 [2]上海市口腔病防治院口腔综合科,上海200001

出　　处：《口腔疾病防治》2018年第10期639-643,共5页Journal of Prevention and Treatment for Stomatological Diseases

基　　金：广东省医学科学技术研究基金项目(A2015215)

摘　　要：目的探讨Twist和Vimentin蛋白在口腔鳞状细胞癌(oral squamous cell carcinoma, OSCC)中的表达情况及其临床意义。方法收集85例OSCC标本和15例正常口腔黏膜组织标本,应用免疫组化SP法检测在标本中Twist和Vimentin蛋白的表达水平,分析其与口腔鳞癌患者临床病理因素的相关性。结果 Twist蛋白在正常组织中的阳性率为13.3%(2/15),在OSCC组织的阳性率为80.0%(68/85),两组相比差异具有统计学意义(χ~2=26.98,P <0.001),同时其在OSCC中的表达与性别(χ~2=0.23,P=0.63)、年龄(χ~2=0.31,P=0.58)、部位(χ~2=1.46,P=0.23)、组织学分化(χ~2=1.52,P=0.47)等因素无关,与临床分期(χ~2=5.40,P=0.02)、淋巴结转移(χ~2=8.35,P=0.006)等因素有关;Vimentin蛋白在正常组中的阳性率为6.7%(1/15),在OSCC组中为74.1%(63/85),两组差异有统计学意义(χ~2=20.71,P <0.001),同时其表达与性别(χ~2=0.40,P=0.53)、年龄(χ~2=0.17,P=0.68)、部位(χ~2=0.74,P=0.39)、组织学分化(χ~2=4.58,P=0.10)等因素无关,与临床分期(χ~2=4.51,P=0.034)、淋巴结转移(χ~2=6.75,P=0.009)等因素有关。Spearman等级相关分析显示,Twist与Vimentin蛋白存在正相关(r=0.578,P<0.05)。结论在OSCC中Twist和Vimentin蛋白表达增加,并且两者存在正相关关系,提示在OSCC中两者可能共同参与肿瘤的发生和发展。Objective To investigate the levels of the Twist and Vimentin proteins in oral squamous cell carcinoma （OSCC） and analyze the clinical significance of Twist and Vimentin.Methods Eighty-five samples of OSCC and fifteen samples of normal oral mucosa were collected. Immunohistochemistry （SP method） was used to detect the expression of proteins, including Twist and vimentin. The relationship among these proteins and clinical pathological parameters was analyzed using SPSS statistical software.Results In the normal group, 13.3% （2/15） of samples were positive for the Twist protein; this value was significantly lower than that in OSCC group （80.0%, 66/85） （χ 2=26.98, P 〈 0.001）. The expression of Twist was associated with clinical stage （χ 2=5.40, P=0.02） and lymph node metastasis （χ 2=8.35, P=0.006）, while no correlations were found between the expression of Twist and sex （χ 2=0.23, P=0.63）, age （χ 2= 0.31, P=0.58）, location （χ 2=1.46, P=0.235） or degree of differentiation （χ 2=1.52, P=0.47）. Additionally, 6.7% of samples （1/15） were positive for vimentin; this value was significantly lower than that in OSCC group （74.1%, 63/85） （χ 2=20.71, P 〈 0.001）. The expression of vimentin was associated with clinical stage （χ 2=4.51, P=0.034） and lymph node metastasis （χ 2=6.75, P=0.009）, while no correlations were found between the expression of vimentin and sex （χ 2=0.40, P=0.53）, age （χ 2=0.17, P=0.68）, location （χ 2=0.74,P=0.39） or degree of differentiation （χ 2=4.58, P=0.10）. Spearman correlation analyses showed that Twist protein expression was positively correlated with vimentin （r=0.578, P〈0.05）. Conclusion Our data demonstrate that in OSCC, Twist and vimentin levels were upregulated, and Twist protein expression was positively correlated with vimentin, which indicates that both Twist and vimentin may be involved in the occurrence of OSCC.

关 键 词：口腔鳞癌 TWIST蛋白 Vimentin蛋白 上皮间质转化 免疫组化 

分 类 号：R783.2[医药卫生—口腔医学]