Effect of TongFengNing Decoction on Uric Acid Levels and Xanthine Oxidase Activity in Hyperuricemia Rats  

作　　者：Jian-hui Wang Jie-mei Guo Bao-lin Li Fang-zhou Teng Ya-ju Zhu Jian-ping Lin Yan Xiao Xiao Mao Lu-lu Huang You-xin Su 

机构地区：[1]Fujian University of Traditional Chinese Medicine, Fujian, China. [2]Fujian Health College, Fujian, China. [3]The Affiliated Rehabilitation Hospital of Fujian University of Traditional Chinese Medicine, Fujian, China.

出　　处：《TMR Modern Herbal Medicine》2018年第4期189-197,共9页TMR现代中药

摘　　要：Objective To observe the effect of TongFengNing Decoction （TD） on uric acid levels, xanthine oxidase （XOD） activity, and XOD mRNA expression of hyperuricemia （HUA） model rats. Methods： 90 rats were randomly divided into 6 groups （n=15）, and the HUA model in all groups except the blank group was established by administering hypoxanthine （HX） by gavage and injecting potassium oxonate （OAPS） intraperitoneally. Rats in all TD groups and allopurinol group were administered multiple doses of TD and a single dose of allopurinol by gavage twice daily for 21 days, while the blank group and the model group were administered normal saline. On the 7th, 14th, and 21st days of drug intervention, serum uric acid （SUA）, urine uric acid （UUA）, intestinal uric acid （IUA）, as well as XOD activity and mRNA expression in the liver and small intestine were measured in randomly selected 5 rats of each group. Results： On the 14th and 21st days of intervention, all TD dose groups and the allopurinol group showed decreased SUA and IUA levels, increased UUA levels, as well as decreased XOD activity and mRNA expression in the liver and small intestine, compared with the model group （P 〈 0.05）. The low- and high-dose TD group and the allopurinol group showed increased SUA and IUA levels, as well as XOD activity and mRNA expression in the liver and small intestine, and decreased UUA levels, compared with the moderate-dose TD group （P〈0.05）. Upon extending the drug intervention time of each TD dose group, SUA and IUA levels, XOD activity, and XOD mRNA expression in the liver and small intestine decreased and UUA levels increased （P 〈 0.05）. Conclusion： TD reduces SUA levels in HUA model rats, which promotes uric acid excretion and inhibits XOD activity and XOD mRNA expression to reduce uric acid production. The reduction in uric acid level by the intermediate dose of TD was better than that by allopurinol and the low and high doses of TD.目的 观察痛风宁对 HUA 大鼠尿酸、黄嘌呤氧化酶（xanthine oxidase,XOD）活性及 XOD mRNA 的影响. 方法： 90只 SD大鼠随机分为 6组（每组15只）.除空白组外,各组大鼠均以次黄嘌呤（HX）灌胃和氧嗪酸钾（OAPS）腹腔注射建立 HUA 大鼠模型.低、中、高剂量痛风宁组、别嘌醇组大鼠分别用不同剂量的相应药液灌胃,空白组与模型组用等量生理盐水灌胃,2次/d,共21d.在干预后的第7 、14 、21 d每组随机取5只大鼠,分别测定血尿酸（serum uric acid,SUA）、尿尿酸（urine uric acid,UUA）、肠道尿酸（Intestines uric acid,IUA）以及肝脏、小肠组织 XOD活性及其 mRNA表达情况. 结果： 干预第14 天和第21天,各剂量痛风宁组和别嘌呤醇组与模型组相比均显示 SUA、 IUA水平降低,UUA 水平升高,肝脏和小肠组织内 XOD 活性和XOD mRNA 表达降低（P 〈 0.05）.低、高剂量痛风宁组和别嘌呤醇组与中剂量痛风宁组相比,SUA、IUA水平升高,肝脏和小肠组织内 XOD活性和 XOD mRNA表达增加,UUA 水平降低（P 〈 0.05）.随着各剂量痛风宁组药物干预时间延长,SUA、IUA 水平、肝脏和小肠组织内 XOD活性、XOD mRNA 表达持续降低,UUA水平升高（P 〈 0.05）. 结论： 痛风宁能降低HUA模型大鼠SUA,这可能与其促进尿酸排泄,同时可抑制XOD活性并干预XOD mRNA的表达以减少尿酸生成有关.其中痛风宁中剂量降尿酸效果优于低、高剂量及别嘌醇.

关 键 词：TongFengNing HYPERURICEMIA Xanthine oxidase Uric acid Traditional Chinese medicine 

分 类 号：R542[医药卫生—心血管疾病]