联合免疫组化检测不同类型的输卵管浆液性上皮病变的发生情况  

Detection of Different Types of Serpingous Serous Epithelial Lesions by Combined Immunohistochemistry

作　　者：桂照华[1] 吴景[1] 程修强 何杰[1] GUI Zhao-hua;WU Jing;CHENG Xiu-qiang;HE Jie(Department of Pathology,Anhui Turnout Ho spital,Hefei 230031,China)

机构地区：[1]安徽省肿瘤医院病理科,安徽合肥230031

出　　处：《标记免疫分析与临床》2018年第9期1322-1325,1330,共5页Labeled Immunoassays and Clinical Medicine

摘　　要：目的观察卵巢浆液性肿瘤中输卵管上皮的病理形态学改变.初步探讨输卵管上皮病变与卵巢浆液性癌发生的关系。方法对卵巢39例高级别浆液性癌、7例低级别浆液性癌、10例交界性浆液性肿瘤的输卵管按照广泛逐层切取检查法取材和切片,并用全自动免疫组化仪检测P53表达,观察输卵管上皮P53印记细胞、浆液性上皮内癌和浸润性浆液性癌的发生情况。结果 P53印记和STIC仅出现在卵巢高级别浆液性癌组,其发生率分别为33. 3%和10. 3%;SIC在卵巢高级别浆液性癌的发生率(64.1%)显著高于低级别浆液性癌(25.0%)(P><0.05)。13例存在P53印记的病例中,共计发现20个病灶,位于输卵管伞端15个,位于壶腹部5个;4例出现STIC的病例中共计发现6个病灶,均位于伞端。HGSC组输卵管上皮内癌和浸润性癌P53均呈强阳性或阴性表达,而LGSC组浸润性呈弱阳性表达。结论输卵管上皮浆液性病变与卵巢高级别浆液性癌的发生有一定关系,P53免疫组织化学染色对于识别这些输卵管病变有帮助。Objective To observe the histopathological change of oviduct epithelium in ovarian serous tumor. Methods 39 cases of high level high-grade serous carcinoma,7 cases of low-grade serous carcinoma and 10 cases of borderline serous tumor were collected. Sections from fallopian tubes were selected in accordance with the wide layer cut test method. Expression of P53 was detected by the automatic and immunohistochemical detection. P53 positive oviduct epithelial cells and the occurrence of serous intraepithelial carcinoma and invasive serous carcinoma were observed. Results （ 1 ） P53 and STIC mark only appeared in the ovarian high- grade serous carcinoma group, and the incidence rates were 33.3 % and 10.3 %. SIC in the incidence of high-grade ovarian serous carcinoma （64.1% ） was significantly higher than that of low-grade serous carcinoma（25% ）（P 〈 0.05）. （2）Among 3 cases with positive P53, a total of 20 lesions were observed, with 15 cases in the fallopian tube and 5 cases in ampulla. 4 cases of STIC cases within a total of 6 lesions were found, which were located in the fimbria; （3） HGSC group of tubal intraepithelial carcinoma and invasive carcinoma showed strong P53 positive or negative expression, and invasive group LGSC weakly positive expression. Conclusion The serous lesion of oviduct is related to the occurrence of advanced serous carcinoma of the ovary. P53 immunohistochemical staining is helpful for identifying these fallopian tube diseases.

关 键 词：P53印记细胞 卵巢浆液性肿瘤 输卵管上皮病变 免疫组织化学 

分 类 号：R737.31[医药卫生—肿瘤]