硫磺菌多糖的分离纯化及体外抗氧化、抗肿瘤活性初探  

Isolation,Purification,Antioxidant and Antitumor Activities of Polysaccharides from Laetiporus sulphureus

作　　者：胡亚平[1] 王锦[1] 郑丽[1] 田新利[1] 代玲[1] 林玲辉[1] 王立安[2] HU Ya-ping;WANG Jin;ZHENG Li;TIAN Xin-li;DAI Ling;LIN Ling-hui;WANG Li-an(Xingtai Medical College,Xingtai 054000,China;College of life sciences,Hebei Normal University,Shijiazhuang 050024,China)

机构地区：[1]邢台医学高等专科学校,河北邢台054000 [2]河北师范大学生命科学学院,河北石家庄050024

出　　处：《中国现代中药》2018年第9期1131-1136,共6页Modern Chinese Medicine

基　　金：河北省科技计划重点项目(16237301D);河北省现代农业产业技术体系创新团队项目(HBCT2018050207);河北省高等学校科学技术研究项目(ZC2016059);邢台市科技计划项目(2015ZC167);河北师范大学科技类技术创新基金(L2017K03)

摘　　要：目的:将硫磺菌子实体粗多糖进行分离纯化,对纯化产物进行理化特性和抗氧化抗肿瘤活性的检测。方法:硫磺菌子实体干粉采用水提醇沉、Sevage法去蛋白制备得粗多糖,采用DEAE-52色谱柱分离纯化粗多糖,通过扫描电镜(SEM)、红外光谱(IR)和氢核磁共振谱(1H-NMR)对纯化后的多糖进行检测,采用清除DPPH自由基和MTT法检测多糖活性。结果:硫磺菌子实体分离得到三种多糖组分(LSPS-Ⅰ、LSPS-Ⅱ、LSPS-Ⅲ)。LSPS-Ⅰ和LSPS-Ⅱ多糖的含量分别为85. 43%和80. 12%; LSPS-Ⅰ呈现片状结构,LSPS-Ⅱ呈现不规则多面体结构; LSPS-Ⅰ和LSPS-Ⅱ均具有糖类物质的吸收峰且为α构型; LSPS-Ⅰ和LSPS-Ⅱ均表现出较强的清除DPPH自由基活性,EC50值分别为10. 74、15. 67 mg·m L^(-1)。LSPS-Ⅱ对人胃癌细胞株BGC的增殖有较强的抑制作用,IC50值为848μg·m L^(-1)。结论:硫磺菌子实体粗多糖的分离纯化产物LSPS-Ⅰ和LSPS-Ⅱ均有较强的抗氧化活性,LSPS-Ⅱ还有一定的抗胃癌细胞作用。Objective： To isolate and purify the total polysaccharides extracted from Laetiporus sulphureus fruit body. The physicochemical properties and antioxidant and antitumor activities of the purified products were detected. Methods：Using L. sulphureus fruit body as material,the crude polysaccharide was extracted by using water extraction and alcohol precipitation preparation,protein was removed by Sevage method,the purified polysaccharide was obtained through the DEAE-52 column. The purified polysaccharide was detected by scanning electron microscopy（ SEM）,infrared spectroscopy（ IR） and hydrogen nuclear magnetic resonance（1 H-NMR）. The activity of polysaccharides was detected by scavenging DPPH radical and MTT. Results： Three components（ LSPS-I,LSPS-II and LSPS-III） were obtained from L. sulphureus fruit body. The content of polysaccharide from LSPS-I and LSPS-II were 85. 43% and 80. 12%,respectively. LSPS-I showed a lamellar structure,LSPSII showed irregular polyhedron structure. LSPS-I and LSPS-II had carbohydrate absorption peak and alpha configuration. LSPS-I and LSPS-II had strong activity of scavenging DPPH free radicals,and EC50 values were 10. 74 and 15. 67 mg·m L-1,respectively. Antitumor results showed that LSPS-II had a strong inhibitory effect on the proliferation of human gastric cancer cell line BGC,and the IC50 value was 848 μg·m L-1. Conclusion： LSPS-I and LSPS-II had strong antioxidant activity. LSPS-II also had certain anti gastric cancer effect.

关 键 词：硫磺菌多糖 DPPH自由基 抗氧化 人胃癌细胞株BGC 抗肿瘤 

分 类 号：R285[医药卫生—中药学] R284.2[医药卫生—中医学]