血糖升高速度对糖尿病大鼠脑组织基质金属蛋白酶-9水平的影响  

Influence of glucose rising velocity on matrix metalloproteinase-9 levels of brain tissue in diabetic rats

作　　者：余爱勇 赵迎春[1] 赵玉武[2] 潘晓春[1] YU Ai-yong;ZHAO Ying-chun;ZHAO Yu-wu(Department of Neurology,Shanghai Son~iang District Central Hospital,Shanghai 201699,China)

机构地区：[1]上海市松江区中心医院神经内科,201699 [2]上海交通大学附属第六人民医院神经内科

出　　处：《临床神经病学杂志》2018年第5期359-362,共4页Journal of Clinical Neurology

基　　金：上海市松江区科学技术攻关项目(15SJGG30)

摘　　要：目的探讨不同的血糖升高速度对糖尿病大鼠脑组织基质金属蛋白酶-9(MMP-9)水平的影响。方法将30只SD大鼠随机分为空白对照组(Blank组)、假手术组(Sham组)、快速组(Speed(1)组)、较快组(Speed(2)组)、较慢组(Speed(3)组)和慢速组(Speed(4)组),每组5只大鼠。将大鼠制作成糖尿病模型。Blank组大鼠股静脉注射0. 9%氯化钠液,维持血糖在(5. 50±0. 25) mmol/L。Sham组大鼠股静脉注射25%葡萄糖和15 U/kg的胰岛素1. 5 ml/h,维持血糖在(5. 50±0. 25) mmol/L。其余4组大鼠股静脉泵入胰岛素1U/h诱导低血糖并维持1 h,然后泵入25%葡萄糖将血糖升高至5～6 mmol/L并维持1. 5 h。取大脑皮质、海马、丘脑、脑干、小脑脑组织,采用免疫印迹法检测MMP-9蛋白的表达。结果在皮质、海马、丘脑组织中,Speed(1)组和Speed(4)组的前体-MMP-9水平明显高于Sham组和Blank组(均P <0. 05)。在皮质、海马、丘脑组织中,Speed(1)组和Speed(4)组的活性MMP-9水平明显高于Blank组、Sham组、Speed(2)组和Speed(3)组(均P <0. 05); Speed(2)组和Speed(3)组的活性MMP-9水平明显高于Blank组和Sham组(均P <0. 05)。结论低血糖后任何速度的葡萄糖再灌注都会带来脑损伤,但合理的控制葡萄糖再灌注速度或许可减轻脑损伤。Objective To discuss the influence of different glucose rising velocity on the matrix metalloproteinase-9 （MMP-9） levels of brain tissue in diabetic rats. Methods Thirty diabetic SD rats were divided into Blank group, Sham group, Fast group （Speed① group） , Faster group （Speed② group） , Slower group （ Speed ③ group） and Slow group （Speed④ group）. The rats in the Blank group were injected with 0. 9% sodium chloride solution through the femoral vein to maintain blood glucose at （ 5.50 ± 0.25 ） mmol/L. Rats in the Sham group were injected with 25% glucose and 15 U/kg of insulin via the femoral vein at a rate of 1.5 ml/h to maintain blood glucose at （ 5.50 ± 0.25 ） mmol/L. The remaining 4 groups of rats received 1 U/h of insulin pumped into the femoral vein to induce hypoglycemia for l h, and then 25% glucose solution was pumped to raise the blood glucose to 5 -6 mmol/L for 1.5 h. The expression of MMP-9 protein was detected by immunoblotting in cerebral cortex, hippocampus, thalamus, brainstem and cerebellum. Results The concentrations of Pro-MMP-9 in cortex, hippocampus, and thalamus of Speed① group and Speed④ group were significantly higher than those in Sham group and Blank group （ all P 〈 0. 05 ）. The concentrations of Activated MMP-9 in cortex, hippocampus, and thalamus of Speed① group and Speed④ group were significantly higher than those in Sham group, Blank group, Speed② group and Speed③ group （ all P 〈 0. 05 ） ; And the concentrations of Activated MMP-9 in cortex, hippocampus, and thalamus of Speed② group and Speed③ group were significantly higher than those in Sham group and Blank group （ all P 〈 0. 05 ）. Conclusion Any rate of glucose reperfusion after hypoglycemia can cause brain damage, but a reasonable control of glucose reperfusion speed may reduce brain damage.

关 键 词：血糖升高速度 脑损伤 基质金属蛋白酶-9 

分 类 号：R741.05[医药卫生—神经病学与精神病学]