Anti-parasitic effects of water-soluble alkaloid fractions from ethanolic extracts of Sophora moorcroftiana seeds in Caenorhabditis elegans  

作　　者：LUO Yan-Ping ZHANG Yuan ZHANG Hui-Min ZHANG Hong ZHANG Lin YU Hong-Juan CAO Ming-Qiang SHI Yan-Bin ZHI De-Juan MA Xing-Ming DONG Kai-Zhong 

机构地区：[1]Department of Immunology, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China [2]Key Lab of Preelinical Study for New Drugs of Gansu Province, Lanzhou 730000, China [3]School of Pharmacy, Lanzhou University, Lanzhou 730000, China [4]Department of Microbiology, Medical College, Northwest Minzu University, Lanzhou 730000, China

出　　处：《Chinese Journal of Natural Medicines》2018年第9期665-673,共9页中国天然药物（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.31360604);Lanzhou University Innovation and Entrepreneurship Fund for College Students(No.2015073001362)

摘　　要：Parasite infections of humans and animals remain a major global health problem, with limited choice of drugs being available to the treatment of parasitosis in the clinic. Sophora moorcroftiana(S. moorcroftiana) is a shrub that grows in Tibet Plateau of China. Decoction of the seeds has been used as a traditional Tibetan medicine to treat parasitosis for years. But the anti-parasitic effects of water-soluble fractions in the seeds need further investigation. In the present study, the water-soluble alkaloid fractions(E2) were obtained from S. moorcroftiana seeds by refluxing extraction with 60% ethanol and low polarity fraction(E2-a) and high polarity fraction(E2-b) were subsequently isolated from E2 using column chromatography. As a parasite model, Caenorhabditis elegans(C. elegans) were treated with different fractions and their survivals were recorded. The results showed that that E2-a induced a lower survival rate in C. elegans than E2-b and E2. The protoscoleces of Echinococcus granulosus(E. granulosus) were cultured in the presence of E2-a. Compared with E2-b and E2, protoscoleces exhibited decreased survival rate following E2-a treatment. Furtherly, the effects of E2-a on the behavior, brood size, and lifespan of the worms were investigated. Body bend frequencies of the worms treated with the high concentration of E2-a were reduced by two-thirds compared with the control group(P < 0.01). Compared with non-E2-a-treated group, exposure of nematodes to E2-a led to a decrease in head thrashes and pharyngeal pumps frequency(P < 0.01). E2-a treatment resulted in a significantly lower brood size(P < 0.01). Additional E2-a treatment induced a significantly shortened lifespan, compared with the control(P < 0.05). These findings indicated that water-soluble fraction E2-a from S. moorcroftiana seeds was a potential helminthic agent.Parasite infections of humans and animals remain a major global health problem, with limited choice of drugs being available to the treatment of parasitosis in the clinic. Sophora moorcroftiana（S. moorcroftiana） is a shrub that grows in Tibet Plateau of China. Decoction of the seeds has been used as a traditional Tibetan medicine to treat parasitosis for years. But the anti-parasitic effects of water-soluble fractions in the seeds need further investigation. In the present study, the water-soluble alkaloid fractions（E2） were obtained from S. moorcroftiana seeds by refluxing extraction with 60% ethanol and low polarity fraction（E2-a） and high polarity fraction（E2-b） were subsequently isolated from E2 using column chromatography. As a parasite model, Caenorhabditis elegans（C. elegans） were treated with different fractions and their survivals were recorded. The results showed that that E2-a induced a lower survival rate in C. elegans than E2-b and E2. The protoscoleces of Echinococcus granulosus（E. granulosus） were cultured in the presence of E2-a. Compared with E2-b and E2, protoscoleces exhibited decreased survival rate following E2-a treatment. Furtherly, the effects of E2-a on the behavior, brood size, and lifespan of the worms were investigated. Body bend frequencies of the worms treated with the high concentration of E2-a were reduced by two-thirds compared with the control group（P 〈 0.01）. Compared with non-E2-a-treated group, exposure of nematodes to E2-a led to a decrease in head thrashes and pharyngeal pumps frequency（P 〈 0.01）. E2-a treatment resulted in a significantly lower brood size（P 〈 0.01）. Additional E2-a treatment induced a significantly shortened lifespan, compared with the control（P 〈 0.05）. These findings indicated that water-soluble fraction E2-a from S. moorcroftiana seeds was a potential helminthic agent.

关 键 词：SOPHORA moorcroftiana ALKALOIDS ANTHELMINTICS CAENORHABDITIS elegans Protoscoleces 

分 类 号：R965[医药卫生—药理学]