Discoursing on Soxhlet extraction of ginseng using association analysis and scanning electron microscopy  

作　　者：Yuan Yue Zi-Dong Qiu Xian-You Qu Ai-Ping Deng Yuan Yuan Lu-Qi Huang Chang-Jiang-Sheng Lai 

机构地区：[1]Chongqing Academy of Chinese Materia Medica,Chongqing Key Laboratory of Traditional Chinese Medicine Resources,Chongqing Sub-center of National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences,Chongqing 400065,PR China [2]National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences,State Key Laboratory Breeding Base of Dao-di Herbs,Beijing 100700,PR China [3]State Key Laboratory of Natural Medicines(China Pharmaceutical University),No.24 Tongjia lane,Nanjing 210009,PR China

出　　处：《Journal of Pharmaceutical Analysis》2018年第5期312-317,共6页药物分析学报（英文版）

基　　金：financial support of the key project at the central government level:The Ability Establishment of Sustainable Use for Valuable Chinese Medicine Resources(No.2060302);the Independent Research Grant of National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences(No.ZZXT201608);the National Natural Science Foundation of China(No.81603293)

摘　　要：Soxhlet extraction is a common method of sample preparation. However, there has been no discussion about the efficiency of Soxhlet extraction from different batches and the factors that cause content fluctuation. In this study, Panax ginseng was selected as a model sample. Soxhlet extraction by means of a water bath, which has always been neglected, was identified as a novel key factor in the poor repeat- ability in different batches of Soxhlet extraction, as it can affect the siphon times and reflux time, which have been positively correlated with the ginsenoside contents. By substituting round bottom flasks in the same column, the relative standard deviation of the most fluctuated compound, ginsenoside Rbl, was decreased from 24.6% to 5.02%. Scanning electron microscopy analysis confirmed that the breakdown of the surface of the ginseng powder in the Soxhlet extraction led to a better dissolution of ginsenosides, indicating that chloroform may promote the extraction of ginsenosides by disrupting the cell structure. Moreover, 70% methanol was regarded as the better solvent for extracting the ginsenosides. Overall, this work offers a practical and effective protocol for improving the accuracy and repeatability of Soxhlet extraction methodology for ginsenosides and other analytes.Soxhlet extraction is a common method of sample preparation. However, there has been no discussion about the efficiency of Soxhlet extraction from different batches and the factors that cause content fluctuation. In this study, Panax ginseng was selected as a model sample. Soxhlet extraction by means of a water bath, which has always been neglected, was identified as a novel key factor in the poor repeat- ability in different batches of Soxhlet extraction, as it can affect the siphon times and reflux time, which have been positively correlated with the ginsenoside contents. By substituting round bottom flasks in the same column, the relative standard deviation of the most fluctuated compound, ginsenoside Rbl, was decreased from 24.6% to 5.02%. Scanning electron microscopy analysis confirmed that the breakdown of the surface of the ginseng powder in the Soxhlet extraction led to a better dissolution of ginsenosides, indicating that chloroform may promote the extraction of ginsenosides by disrupting the cell structure. Moreover, 70% methanol was regarded as the better solvent for extracting the ginsenosides. Overall, this work offers a practical and effective protocol for improving the accuracy and repeatability of Soxhlet extraction methodology for ginsenosides and other analytes.

关 键 词：GINSENG GINSENOSIDES Soxhlet extraction REPEATABILITY Siphon times 

分 类 号：R284.2[医药卫生—中药学]