FOXC1基因对肺癌A549细胞株生物学特性影响  

EFFECT OF FORKHEAD BOX C1 GENE SILENCING ON THE BIOLOGICAL CHARACTERISTICS OF LUNG CANCER A549 CELL LINE

作　　者：李立明 张秋莹 栗四方 花光斌 LI Liming;ZHANG Qiuying;LI Sifang;HUA Guangbin(Jiaozuo Coal Industry（Group）Co.,Ltd.Central Hospital,Jiaozuo 454000,China)

机构地区：[1]焦作煤业(集团)有限责任公司中央医院,河南焦作454000

出　　处：《青岛大学学报（医学版）》2018年第5期519-523,527,共6页Journal of Qingdao University(Medical Sciences)

基　　金：河南省科技发展计划项目(152300410158)

摘　　要：目的探讨沉默叉头框蛋白C1(FOXC1)基因对肺癌A549细胞株生物学特性的影响。方法培养人肺癌A549细胞,随机分为siRNA-FOXC1组、错义siRNA组和空白对照组,应用实时荧光定量PCR技术检测细胞中FOXC1基因表达,Western blot法检测细胞中FOXC1蛋白表达,CCK-8法检测细胞增殖能力,流式细胞术检测细胞凋亡率,划痕实验检测细胞迁移能力,Transwell法检测细胞侵袭能力。结果siRNA-FOXC1组细胞FOXC1mRNA和蛋白相对表达量均低于错义siRNA组和空白对照组,差异均有显著性(F=2 521.827、37.226,P<0.05);siRNA-FOXC1组48、72和96h时细胞存活率均低于错义siRNA组和空白对照组,差异均有显著性(F=22.857～103.502,P<0.05);siRNA-FOXC1组细胞凋亡率高于错义siRNA组和空白对照组,差异有显著性(F=25.844,P<0.05)。划痕实验显示,siRNA-FOXC1组24h后划痕愈合率低于错义siRNA组和空白对照组,差异有显著性(F=70.260,P<0.05)。Transwell实验显示,siRNA-FOXC1组侵袭细胞数低于错义siRNA组和空白对照组,差异有显著性(F=95.879,P<0.05)。结论特异性沉默人肺癌A549细胞中FOXC1基因表达可减少细胞增殖,促进细胞凋亡,有效抑制细胞迁移及侵袭能力。Objective To investigate the effect of forkhead box C1 （ FOXC1 ） gene silencing on the biological characteristics of lung cancer A549 cell line. Methods Human lung cancer A549 cells were cultured and randomly divided into siRNA-FOXC1 group, scramble siRNA group, and blank control group. Quantitative real-time PCR and Western blot were used to measure the mRNA and protein expression of FOXC1 in cells; CCK-8 assay was used to measure cell proliferation; flow cytometry was used to measure cell apoptosis rate; wound healing assay was used to evaluate cell migration ability; Transwell assay was used to evaluate cell invasion ability. Results The siRNA-FOXC1 group had significantly lower mRNA and protein expression of FOXC1 than the scramble siRNA group and the blank control group （ F =2 521.827,37.226; P 〈0.05）. The siRNA-FOXC1 group had significantly lower survival rates of cells at 48,72, and 96 h than the scramble siRNA group and the blank control group （ F = 22.857- 103.502, P 〈0.05）. The siRNA-FOXC1 group had a significantly higher apoptosis rate than the scramble siRNA group and the blank control group （ F =25.844, P 〈0.05）. The wound healing assay showed that after 24 h, the siRNA-FOXC1 group had a significantly lower wound healing rate than the scramble siRNA group and the blank control group （ F =70.260, P 〈 0.05 ）. The Transwell assay showed that the siRNA-FOXC1 group had a lower number of invasive cells than the scramble siRNA group and the blank control group （ F =95.879, P 〈0.05）. Conclusion Specific silencing of the FOXC1 gene in human lung cancer A549 cells can reduce cell proliferation, promote cell apoptosis, and effectively inhibit cell migration and invasion abilities.

关 键 词：癌 非小细胞肺 叉头框蛋白C1 基因沉默 细胞增殖 肿瘤浸润 

分 类 号：R730.26[医药卫生—肿瘤]