IGF-1R对乳腺癌细胞株MDA-MB231细胞增殖及迁移能力的影响  

Effects of recombinant plasmid of p EGFP-N1-IGF-1R on proliferation and migration of MDA-MB231 cells

作　　者：杨晓民 贺赛[1] 宋张骏 王虎霞 丁艳妮 陈楠 马冬梅 Yang Xiaomin;He Sai;Song Zhangjun;Wang Huxia;Ding Yanni;Chen Nan;Ma Dongmei(Breast Disease Center,Shaanxi Province Tumor Hospital,Shaanxi Xi＇an 710061,China;Breast Surgery;Department of Orthopedics,the First Affiliated Hospital,Medical School of Xi＇an Jiaotong University,Shaanxi Xi＇an 710061,China)

机构地区：[1]陕西省肿瘤医院乳腺中心,陕西西安710061 [2]西安交通大学医学院第一附属医院乳腺外科,陕西西安710061 [3]西安交通大学医学院第一附属医院骨科,陕西西安710061

出　　处：《现代肿瘤医学》2018年第22期3550-3553,共4页Journal of Modern Oncology

基　　金：陕西省科技厅社会发展攻关计划项目(编号:2013K12-03-17)

摘　　要：目的:研究IGF-1R基因对乳腺癌细胞株MDA-MB231细胞增殖及迁移能力的影响。方法:将本课题组前期构建的质粒p EGFP-N1-IGF-1R转染入MDA-MB231细胞系,采用Western blot法和Realtime PCR法检测IGF-1R蛋白与mRNA的表达情况;通过MTT法和Transwell法检测p EGFP-N1-IGF-1R对MDA-MB231细胞增殖及迁移侵袭能力的影响。结果:p EGFP-N1-IGF-1R转染入MDA-MB231细胞后,其IGF-1R的表达水平明显上调;与空白对照组和空载体组相比,过表达组细胞增殖能力明显增强(P <0. 05),细胞迁移能力增强(P <0. 05)。结论:IGF-1R基因能够增强MDA-MB231细胞的增殖能力及迁移能力。Objective： To explore the effects of p EGFP-N1-IGF-1 R on proliferation and migration of MDA-MB231 cells. Methods： The p EGFP-N1-IGF-1 R was transfected into MDA-MB231 cells. The expression of IGF-1 R gene was detected by Real-time PCR and Western blot. The effects of proliferation and migration of MDA-MB231 cells were examined by MTT and Transwell assay respectively. Results： The MDA-MB231 cells transfected with p EGFP-N1-IGF-1 R plasmid expressed a higher level of IGF-1 R mRNA and protein steadily. The capabilities of proliferation and migration of MDA-MB231 cells in p EGFP-N1-IGF-1 R/MDA-MB231 group were increased significantly compared with MDA-MB231 group and p EGFP-N1/MDA-MB231 group（ P〈0. 05）. Conclusion： IGF-1 R gene can enhance the abilities of proliferation and migration of MDA-MB231 cells.

关 键 词：MDA-MB231 IGF-1R 增殖 迁移 

分 类 号：R737.9[医药卫生—肿瘤]