HIP1在食管癌中的表达及其临床意义  

Expression of HIP1 in esophageal squamous cell carcinoma and its clinical significance

作　　者：张亚斌[1] 孙盈[2] 孙蕾 孙琳[1] 宋立军[1] 崔光彬[1] Zhang Yabin;Sun Ying;Sun Lei;Sun Lin;Song Lijun;Cui Guangbin(Department of Radiology;Department of Thoracic Surgery,Tangdu Hospital,Fourth Military Medical University,Shaanxi Xi＇an 710038,China.)

机构地区：[1]第四军医大学唐都医院放射科,陕西西安710038 [2]第四军医大学唐都医院胸腔外科,陕西西安710038

出　　处：《现代肿瘤医学》2018年第21期3401-3404,共4页Journal of Modern Oncology

基　　金：第四军医大学唐都医院创新发展基金(编号:2016JCYJ009)

摘　　要：目的:探究亨廷顿蛋白相互作用蛋白1(Huntingtin interacting protein 1,HIP1)基因和蛋白在食管癌组织中的表达及其临床意义。方法:采用免疫组织化学法检测85例食管癌组织及其对应癌旁组织中HIP1蛋白的表达,并分析该表达与食管癌患者临床病理特征的关系。荧光定量PCR及Western blot法检测HIP1基因和蛋白在食管癌及其癌旁组织中的表达。结果:HIP1在食管癌组织中的阳性表达率为91. 8%(78/85),显著高于食管癌旁组织(5. 9%,5/85),差异具有统计学意义(P <0. 001)。统计分析发现,HIP1在病理分级组间差异显著(P=0. 03),而在性别、年龄、TNM分期组间无统计学差异(P> 0. 05)。荧光定量PCR及Western blot结果发现,与食管癌旁组织相比,HIP1基因和蛋白在食管癌组织中高表达。结论:HIP1蛋白可能是食管癌组织和癌旁组织中的差异蛋白,可能是食管癌新的分子标志物。Objective To explore the gene and protein expression of HIP1 and its clinical significance in esophageal squamous cell carcinoma （ESCC）. Methods： HIP1 expression was assessed using immunohistochemical （IHC） in 85 ESCC and adjacent ESCC tissues and the association of HIP1 expression with clinicopathological parameters were also evaluated.qRT-PCR and Western blot were used to detect the gene and protein expression of HIP1 in ESCC and adjacent ESCC tissues. Results： The expression rate of HIP1 in ESCC （91.8%,78/85） showed extremely significance compared with adjacent ESCC tissues （5.9%,5/85） （ P 〈0.001）.HIP1 expression was significantly associated with pathological grading （ P =0.03）,but there were no significant differences with gender,age,TNM stage （ P 〉0.05）.qRT-PCR and Western blot results showed that compared with adjacent ESCC tissues,HIP1 gene and protein were higher expressed in ESCC tissues. Conclusion： HIP1 is a differential protein in cancer tissue and adjacent ESCC tissues and can serve as a new molecular biomarker in ESCC.

关 键 词：HIP1 食管癌 标志物 

分 类 号：R735.1[医药卫生—肿瘤]