利用网络分析预测As_2O_3治疗结直肠癌的靶基因  

Using network analysis to predict the target genes of As_2O_3 in the treatment of colorectal cancer

作　　者：柏金 于若曦 宋慧聪 吕飞 邓明明 陈洋[2] 邹丹 隋丹 郑新宇[1] 张晔[1] Bai Jin;Yu Ruoxi;Song Huicong;Lv Fei;Deng Mingming;Chen Yang;Zou Dan;Sui Dan;Zheng Xinyu;Zhang Ye(Laboratory I of Cancer Institute;Department of Respiratory and Infectious Disease of Geriatrics,The First Hospital of China Medical University,Liaoning Shenyang 110001,China.)

机构地区：[1]中国医科大学附属第一医院肿瘤研究所一室,辽宁沈阳110001 [2]中国医科大学附属第一医院干诊老年病科,辽宁沈阳110001

出　　处：《现代肿瘤医学》2018年第21期3437-3440,共4页Journal of Modern Oncology

基　　金：国家自然科学基金项目(编号:81270036;30901736);辽宁省重点研发计划攻关项目(编号:2017225029);沈阳市科技计划项目(编号:18-014-4-11);中国医科大学附属第一医院科学研究基金(编号:FHCMU-FSR)

摘　　要：目的:探究三氧化二砷(arsenic trioxide,As_2O_3)作用于结直肠癌的靶基因。方法:利用pharm GKB数据库和drugbank5. 0数据库分别提取三氧化二砷的直接靶基因,利用STRING10. 5数据库筛选与三氧化二砷已知靶基因互相作用的蛋白,利用web Gestalt数据库对互作蛋白进行通路分析和GO注释,运用c Bio Protal数据库和The Human Protein Atlas数据库对重点通路的核心基因进行变异和表达分析。结果:pharm GKB数据库和drugbank5. 0数据库共提取出11个直接靶基因,STRING10. 5数据库筛选出384个互作蛋白,web Gestalt数据库的GO注释这384个靶基因主要集中于19类分子功能,在前10条通路中筛选出3条与结直肠癌有关的通路,3个核心基因在结直肠癌中存在点突变和基因缺失,其中NF-κB1和RELA在结直肠癌组织中存在高表达。结论:三氧化二砷可能通过NF-κB信号通路治疗结直肠癌。Objective To explore the target genes of As 2O 3 in therapy of colorectal cancer （CRC）. Methods： The direct target genes were extracted by pharmGKB database and drugbank5.0 database.Direct protein targets associated genes were filtrated by STRING10.5 database.KEGG pathway and GO analysis of interactive protein were operated by webGestalt database.Hub genes from important pathways were analyzed by cBioProtal database and the Human Protein Atlas database. Results： 11 direct target genes were found from pharmGKB and drugbank5.0.384 direct protein targets associated genes were identified for As 2O 3 by STRING10.5.And 19 molecular functions and top 10 pathways were explored by webGestalt.Finally,3 hub genes were found out.Moreover,3 hub genes have mutation and gene deep deletion in CRC,of which NF-κB1 and RELA are highly expressed in CRC tissues. Conclusion： As 2O 3 may be used to treat CRC through the NF-κB signaling pathway.

关 键 词：三氧化二砷 结直肠癌 NF-ΚB信号通路 

分 类 号：R735.35[医药卫生—肿瘤] R735.37[医药卫生—临床医学]