MiR-34a在人鼻咽癌中的表达及临床意义  

Expression and Clinical Significance of miR-34a in Human Nasopharyngeal Carcinoma

作　　者：柳伟 蒋成义[1] 江涛[1] 许亚佳[1] 王斌 

机构地区：[1]蚌埠医学院第一附属医院耳鼻咽喉-头颈外科,安徽蚌埠233004

出　　处：《河北北方学院学报（自然科学版）》2018年第10期5-8,共4页Journal of Hebei North University:Natural Science Edition

基　　金：安徽省高等学校自然科学研究重点项目(No.KJ2018A0996);蚌埠医学院科研面上项目(No.BYKY1772)

摘　　要：目的探讨miR-34a在鼻咽癌中的表达及其临床意义。方法收集30例鼻咽癌组织标本,qRTPCR检测鼻咽癌组织中miR-34a表达量,对miR-34a表达与肿瘤临床病理特征及患者预后相关性进行分析,探讨miR-34a表达是否影响鼻咽癌细胞的迁移及侵袭能力。结果 30例鼻咽癌组织中miR-34a的相对表达量为(2.367±0.235),而对应癌旁组织为(5.399±0.463);其中22例鼻咽癌组织(73.33%)中miR-34a的相对表达量低于对应癌旁组织,差异有统计学意义(P<0.001);存在淋巴结转移及具有较高的TNM分期(Ⅲ+Ⅳ)的鼻咽癌组织中miR-34a的表达更低(P<0.01)。结论 miR-34a在鼻咽癌组织中表达下调,并与患者不良临床病理特征相关,上调miR-34a表达可能会抑制鼻咽癌细胞增殖、迁移和侵袭,促进凋亡,miR-34a基因可能作为一种抑癌基因在鼻咽癌的发生和发展中发挥重要作用。Objective To explore the expression of miR-34a in nasopharyngeal carcinoma and its clinical significance. Methods 30 nasopharyngeal carcinoma tissue samples were collected in the study.The expression quantity of miR-34a in these samples were detected by qRT-PCR.The correlations between expression of miR-34a and the clinicopathological features of tumors,and patients’ prognosis were analyzed to investigate whether miR-34a expression can affect the migration and invasion of nasopharyngeal carcinoma cells. Results The relative expression quantity of 30 nasopharyngeal carcinoma tissue was（2.367±0.235）,while that of corresponding paracancerous tissue was（5.399±0.463）;In 22 cases of nasopharyngeal carcinoma tissue samples（73.33%）,the relative expression of miR-34a was lower than that of corresponding paracancerous tissues （P 〈0.001）;Less expression of miR-34a was found in nasopharyngeal carcinoma tissue with lymph node metastasis and higher TNM stage（Ⅲ＋Ⅳ） （P 〈0.01）. Conclusion The expression of miR-34a in nasopharyngeal carcinoma is down-regulated,which is associated with poor clinical pathological features of patients;increasing the expression of miR-34a may restrain nasopharyngeal carcinoma cell from proliferation,moving and invasion,and accelerate its apoptosis at the meantime.MiR-34a gene may play an important role as a tumor suppressor gene in the occurrence and development of nasopharyngeal carcinoma.

关 键 词：MIR-34A 鼻咽癌 增殖 凋亡 迁移 侵袭 

分 类 号：R739.62[医药卫生—肿瘤] R739.63[医药卫生—临床医学]